Characterizing core microbiota and regulatory functions of the pig gut microbiome
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Characterizing core microbiota and regulatory functions of the pig gut microbiome. / Hu, Jun; Chen, Jianwei; Ma, Libao; Hou, Qiliang; Zhang, Yong; Kong, Xiangfeng; Huang, Xingguo; Tang, Zhonglin; Wei, Hong; Wang, Xiangru; Yan, Xianghua.
I: The ISME Journal, Bind 18, Nr. 1, wrad037, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Characterizing core microbiota and regulatory functions of the pig gut microbiome
AU - Hu, Jun
AU - Chen, Jianwei
AU - Ma, Libao
AU - Hou, Qiliang
AU - Zhang, Yong
AU - Kong, Xiangfeng
AU - Huang, Xingguo
AU - Tang, Zhonglin
AU - Wei, Hong
AU - Wang, Xiangru
AU - Yan, Xianghua
N1 - Publisher Copyright: © The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.
PY - 2024
Y1 - 2024
N2 - Domestic pigs (Sus scrofa) are the leading terrestrial animals used for meat production. The gut microbiota significantly affect host nutrition, metabolism, and immunity. Hence, characterization of the gut microbial structure and function will improve our understanding of gut microbial resources and the mechanisms underlying host-microbe interactions. Here, we investigated the gut microbiomes of seven pig breeds using metagenomics and 16S rRNA gene amplicon sequencing. We established an expanded gut microbial reference catalog comprising 17 020 160 genes and identified 4910 metagenome-assembled genomes. We also analyzed the gut resistome to provide an overview of the profiles of the antimicrobial resistance genes in pigs. By analyzing the relative abundances of microbes, we identified three core-predominant gut microbes (Phascolarctobacterium succinatutens, Prevotella copri, and Oscillibacter valericigenes) in pigs used in this study. Oral administration of the three core-predominant gut microbes significantly increased the organ indexes (including the heart, spleen, and thymus), but decreased the gastrointestinal lengths in germ-free mice. The three core microbes significantly enhanced intestinal epithelial barrier function and altered the intestinal mucosal morphology, as was evident from the increase in crypt depths in the duodenum and ileum. Furthermore, the three core microbes significantly affected several metabolic pathways (such as "steroid hormone biosynthesis," "primary bile acid biosynthesis," "phenylalanine, tyrosine and tryptophan biosynthesis," and "phenylalanine metabolism") in germ-free mice. These findings provide a panoramic view of the pig gut microbiome and insights into the functional contributions of the core-predominant gut microbes to the host.
AB - Domestic pigs (Sus scrofa) are the leading terrestrial animals used for meat production. The gut microbiota significantly affect host nutrition, metabolism, and immunity. Hence, characterization of the gut microbial structure and function will improve our understanding of gut microbial resources and the mechanisms underlying host-microbe interactions. Here, we investigated the gut microbiomes of seven pig breeds using metagenomics and 16S rRNA gene amplicon sequencing. We established an expanded gut microbial reference catalog comprising 17 020 160 genes and identified 4910 metagenome-assembled genomes. We also analyzed the gut resistome to provide an overview of the profiles of the antimicrobial resistance genes in pigs. By analyzing the relative abundances of microbes, we identified three core-predominant gut microbes (Phascolarctobacterium succinatutens, Prevotella copri, and Oscillibacter valericigenes) in pigs used in this study. Oral administration of the three core-predominant gut microbes significantly increased the organ indexes (including the heart, spleen, and thymus), but decreased the gastrointestinal lengths in germ-free mice. The three core microbes significantly enhanced intestinal epithelial barrier function and altered the intestinal mucosal morphology, as was evident from the increase in crypt depths in the duodenum and ileum. Furthermore, the three core microbes significantly affected several metabolic pathways (such as "steroid hormone biosynthesis," "primary bile acid biosynthesis," "phenylalanine, tyrosine and tryptophan biosynthesis," and "phenylalanine metabolism") in germ-free mice. These findings provide a panoramic view of the pig gut microbiome and insights into the functional contributions of the core-predominant gut microbes to the host.
KW - core gut microbes
KW - gene catalog
KW - germ-free mice
KW - gut microbiome
KW - pig
U2 - 10.1093/ismejo/wrad037
DO - 10.1093/ismejo/wrad037
M3 - Journal article
C2 - 38366194
AN - SCOPUS:85185702130
VL - 18
JO - I S M E Journal
JF - I S M E Journal
SN - 1751-7362
IS - 1
M1 - wrad037
ER -
ID: 384493535