Changes in the gene expression profile during spontaneous migraine attacks
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Changes in the gene expression profile during spontaneous migraine attacks. / Kogelman, Lisette J. A.; Falkenberg, Katrine; Buil, Alfonso; Erola, Pau; Courraud, Julie; Laursen, Susan Svane; Michoel, Tom; Olesen, Jes; Hansen, Thomas F.
I: Scientific Reports, Bind 11, Nr. 1, 8294, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Changes in the gene expression profile during spontaneous migraine attacks
AU - Kogelman, Lisette J. A.
AU - Falkenberg, Katrine
AU - Buil, Alfonso
AU - Erola, Pau
AU - Courraud, Julie
AU - Laursen, Susan Svane
AU - Michoel, Tom
AU - Olesen, Jes
AU - Hansen, Thomas F.
PY - 2021
Y1 - 2021
N2 - Migraine attacks are delimited, allowing investigation of changes during and outside attack. Gene expression fluctuates according to environmental and endogenous events and therefore, we hypothesized that changes in RNA expression during and outside a spontaneous migraine attack exist which are specific to migraine. Twenty-seven migraine patients were assessed during a spontaneous migraine attack, including headache characteristics and treatment effect. Blood samples were taken during attack, two hours after treatment, on a headache-free day and after a cold pressor test. RNA-Sequencing, genotyping, and steroid profiling were performed. RNA-Sequences were analyzed at gene level (differential expression analysis) and at network level, and genomic and transcriptomic data were integrated. We found 29 differentially expressed genes between 'attack' and 'after treatment', after subtracting non-migraine specific genes, that were functioning in fatty acid oxidation, signaling pathways and immune-related pathways. Network analysis revealed mechanisms affected by changes in gene interactions, e.g. 'ion transmembrane transport'. Integration of genomic and transcriptomic data revealed pathways related to sumatriptan treatment, i.e. '5HT1 type receptor mediated signaling pathway'. In conclusion, we uniquely investigated intra-individual changes in gene expression during a migraine attack. We revealed both genes and pathways potentially involved in the pathophysiology of migraine and/or migraine treatment.
AB - Migraine attacks are delimited, allowing investigation of changes during and outside attack. Gene expression fluctuates according to environmental and endogenous events and therefore, we hypothesized that changes in RNA expression during and outside a spontaneous migraine attack exist which are specific to migraine. Twenty-seven migraine patients were assessed during a spontaneous migraine attack, including headache characteristics and treatment effect. Blood samples were taken during attack, two hours after treatment, on a headache-free day and after a cold pressor test. RNA-Sequencing, genotyping, and steroid profiling were performed. RNA-Sequences were analyzed at gene level (differential expression analysis) and at network level, and genomic and transcriptomic data were integrated. We found 29 differentially expressed genes between 'attack' and 'after treatment', after subtracting non-migraine specific genes, that were functioning in fatty acid oxidation, signaling pathways and immune-related pathways. Network analysis revealed mechanisms affected by changes in gene interactions, e.g. 'ion transmembrane transport'. Integration of genomic and transcriptomic data revealed pathways related to sumatriptan treatment, i.e. '5HT1 type receptor mediated signaling pathway'. In conclusion, we uniquely investigated intra-individual changes in gene expression during a migraine attack. We revealed both genes and pathways potentially involved in the pathophysiology of migraine and/or migraine treatment.
KW - MITOCHONDRIAL DYSFUNCTION
KW - ION CHANNELS
KW - BLOOD
KW - METAANALYSIS
KW - RIBOFLAVIN
KW - MECHANISMS
KW - HEADACHE
KW - LINKAGE
KW - STRESS
KW - MTOR
U2 - 10.1038/s41598-021-87503-5
DO - 10.1038/s41598-021-87503-5
M3 - Journal article
C2 - 33859262
VL - 11
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 8294
ER -
ID: 271688415