Changes in mouse epidermal DNA methylation during development of squamous cell carcinoma in response to UVR
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Changes in mouse epidermal DNA methylation during development of squamous cell carcinoma in response to UVR. / Meyer, Olivia Luxford; Andersen, Jeppe Dyrberg; Børsting, Claus; Morling, Niels; Andersen, Mikkel Meyer; Wulf, Hans Christian; Philipsen, Peter Alshede; Lerche, Catharina Margrethe.
I: Experimental Dermatology, Bind 33, Nr. 6, e15123, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Changes in mouse epidermal DNA methylation during development of squamous cell carcinoma in response to UVR
AU - Meyer, Olivia Luxford
AU - Andersen, Jeppe Dyrberg
AU - Børsting, Claus
AU - Morling, Niels
AU - Andersen, Mikkel Meyer
AU - Wulf, Hans Christian
AU - Philipsen, Peter Alshede
AU - Lerche, Catharina Margrethe
N1 - Publisher Copyright: © 2024 The Author(s). Experimental Dermatology published by John Wiley & Sons Ltd.
PY - 2024
Y1 - 2024
N2 - Squamous cell carcinoma (SCC) is a common skin cancer, often caused by exposure to ultraviolet radiation (UVR). Recent studies have shown that changes in DNA methylation play a crucial role in the development of cancers. However, methylation patterns of SCC are not well characterised. Identifying biomarkers for the risk of developing SCC could be helpful for early detection and diagnosis and can potentially improve treatment and prevention strategies. This study aimed to investigate methylation changes in the epidermis of mice exposed to UVR for 24 weeks. We examined the DNA methylation levels of 260 199 CpGs using the Illumina Infinium Mouse Methylation BeadChip and studied the epidermis of UVR-exposed and unexposed mice every 4 weeks for 24 weeks (n = 39). We identified CpGs with large differences in methylation levels (β-values) between UVR-exposed and unexposed mice. We also observed differences in the epigenetic age of these mice. We identified CpGs in Rev, Ipmk, Rad51b, Fgfr2, Fgfr3 and Ctnnb1 that may serve as potential biomarkers for SCC risk and could be helpful for the early detection and prevention of SCC. Further investigations are necessary to determine the biological functions and clinical significance of these CpGs.
AB - Squamous cell carcinoma (SCC) is a common skin cancer, often caused by exposure to ultraviolet radiation (UVR). Recent studies have shown that changes in DNA methylation play a crucial role in the development of cancers. However, methylation patterns of SCC are not well characterised. Identifying biomarkers for the risk of developing SCC could be helpful for early detection and diagnosis and can potentially improve treatment and prevention strategies. This study aimed to investigate methylation changes in the epidermis of mice exposed to UVR for 24 weeks. We examined the DNA methylation levels of 260 199 CpGs using the Illumina Infinium Mouse Methylation BeadChip and studied the epidermis of UVR-exposed and unexposed mice every 4 weeks for 24 weeks (n = 39). We identified CpGs with large differences in methylation levels (β-values) between UVR-exposed and unexposed mice. We also observed differences in the epigenetic age of these mice. We identified CpGs in Rev, Ipmk, Rad51b, Fgfr2, Fgfr3 and Ctnnb1 that may serve as potential biomarkers for SCC risk and could be helpful for the early detection and prevention of SCC. Further investigations are necessary to determine the biological functions and clinical significance of these CpGs.
KW - DNA methylation
KW - epidermis
KW - squamous cell carcinoma
KW - UVR exposure
U2 - 10.1111/exd.15123
DO - 10.1111/exd.15123
M3 - Journal article
AN - SCOPUS:85196617260
VL - 33
JO - Experimental Dermatology
JF - Experimental Dermatology
SN - 0906-6705
IS - 6
M1 - e15123
ER -
ID: 396086040