Annexin A7 mediates lysosome repair independently of ESCRT-III
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Annexin A7 mediates lysosome repair independently of ESCRT-III. / Ebstrup, Malene Laage; Sønder, Stine Lauritzen; Fogde, Ditte Louise; Heitmann, Anne Sofie Busk; Dietrich, Tiina Naumanen; Dias, Catarina; Jäättelä, Marja; Maeda, Kenji; Nylandsted, Jesper.
I: Frontiers in Cell and Developmental Biology, Bind 11, 1211498, 2023.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Annexin A7 mediates lysosome repair independently of ESCRT-III
AU - Ebstrup, Malene Laage
AU - Sønder, Stine Lauritzen
AU - Fogde, Ditte Louise
AU - Heitmann, Anne Sofie Busk
AU - Dietrich, Tiina Naumanen
AU - Dias, Catarina
AU - Jäättelä, Marja
AU - Maeda, Kenji
AU - Nylandsted, Jesper
N1 - Publisher Copyright: Copyright © 2024 Ebstrup, Sønder, Fogde, Heitmann, Dietrich, Dias, Jäättelä, Maeda and Nylandsted.
PY - 2023
Y1 - 2023
N2 - Lysosomes are crucial organelles essential for various cellular processes, and any damage to them can severely compromise cell viability. This study uncovers a previously unrecognized function of the calcium- and phospholipid-binding protein Annexin A7 in lysosome repair, which operates independently of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. Our research reveals that Annexin A7 plays a role in repairing damaged lysosomes, different from its role in repairing the plasma membrane, where it facilitates repair through the recruitment of ESCRT-III components. Notably, our findings strongly suggest that Annexin A7, like the ESCRT machinery, is dispensable for membrane contact site formation within the newly discovered phosphoinositide-initiated membrane tethering and lipid transport (PITT) pathway. Instead, we speculate that Annexin A7 is recruited to damaged lysosomes and promotes repair through its membrane curvature and cross-linking capabilities. Our findings provide new insights into the diverse mechanisms underlying lysosomal membrane repair and highlight the multifunctional role of Annexin A7 in membrane repair.
AB - Lysosomes are crucial organelles essential for various cellular processes, and any damage to them can severely compromise cell viability. This study uncovers a previously unrecognized function of the calcium- and phospholipid-binding protein Annexin A7 in lysosome repair, which operates independently of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. Our research reveals that Annexin A7 plays a role in repairing damaged lysosomes, different from its role in repairing the plasma membrane, where it facilitates repair through the recruitment of ESCRT-III components. Notably, our findings strongly suggest that Annexin A7, like the ESCRT machinery, is dispensable for membrane contact site formation within the newly discovered phosphoinositide-initiated membrane tethering and lipid transport (PITT) pathway. Instead, we speculate that Annexin A7 is recruited to damaged lysosomes and promotes repair through its membrane curvature and cross-linking capabilities. Our findings provide new insights into the diverse mechanisms underlying lysosomal membrane repair and highlight the multifunctional role of Annexin A7 in membrane repair.
KW - Annexin A7
KW - endosomal sorting complexes required for transport III (ESCRT-III)
KW - ER-lysosome membrane contact sites (MCSs)
KW - L-Leucyl-L-Leucine O-methyl ester (LLOMe)
KW - lysosomal integrity
KW - lysosomal membrane permeabilization
KW - lysosome membrane repair
KW - organelle repair
U2 - 10.3389/fcell.2023.1211498
DO - 10.3389/fcell.2023.1211498
M3 - Journal article
C2 - 38348092
AN - SCOPUS:85184239642
VL - 11
JO - Frontiers in Cell and Developmental Biology
JF - Frontiers in Cell and Developmental Biology
SN - 2296-634X
M1 - 1211498
ER -
ID: 391672732