Activation of phospholipase A2 by Hsp70 in vitro
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Activation of phospholipase A2 by Hsp70 in vitro. / Mahalka, Ajay K; Code, Christian; Rezaijahromi, Behnam; Kirkegaard, Thomas; Jaattela, Marja; Kinnunen, Paavo.
I: BBA General Subjects, Bind 1808, Nr. 10, 2011, s. 2569-72.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Activation of phospholipase A2 by Hsp70 in vitro
AU - Mahalka, Ajay K
AU - Code, Christian
AU - Rezaijahromi, Behnam
AU - Kirkegaard, Thomas
AU - Jaattela, Marja
AU - Kinnunen, Paavo
N1 - Copyright © 2011 Elsevier B.V. All rights reserved.
PY - 2011
Y1 - 2011
N2 - We recently suggested a novel mechanism for the activation of phospholipase A2 (PLA2), with a (catalytically) highly active oligomeric state, which subsequently becomes inactivated by conversion into amyloid. This process can be activated by lysophosphatidylcholine which promotes both oligomerization and amyloid activation/inactivation. The heat shock protein 70 (Hsp70), has been demonstrated to be able to revert the conversion of a-synuclein and Alzheimer ß-peptide to amyloid fibrils in vitro. Accordingly, we would expect Hsp70 to sustain the lifetime of the active state of the enzyme oligomer by attenuating the conversion of the enzyme oligomers into inactive amyloid. Here we show that Hsp70 activates PLA2 in vitro, in a manner requiring ATP and Mg(2+).
AB - We recently suggested a novel mechanism for the activation of phospholipase A2 (PLA2), with a (catalytically) highly active oligomeric state, which subsequently becomes inactivated by conversion into amyloid. This process can be activated by lysophosphatidylcholine which promotes both oligomerization and amyloid activation/inactivation. The heat shock protein 70 (Hsp70), has been demonstrated to be able to revert the conversion of a-synuclein and Alzheimer ß-peptide to amyloid fibrils in vitro. Accordingly, we would expect Hsp70 to sustain the lifetime of the active state of the enzyme oligomer by attenuating the conversion of the enzyme oligomers into inactive amyloid. Here we show that Hsp70 activates PLA2 in vitro, in a manner requiring ATP and Mg(2+).
KW - Biocatalysis
KW - Enzyme Activation
KW - HSP70 Heat-Shock Proteins
KW - Phospholipases A2
KW - Spectroscopy, Fourier Transform Infrared
U2 - 10.1016/j.bbamem.2011.06.002
DO - 10.1016/j.bbamem.2011.06.002
M3 - Journal article
C2 - 21683684
VL - 1808
SP - 2569
EP - 2572
JO - B B A - General Subjects
JF - B B A - General Subjects
SN - 0304-4165
IS - 10
ER -
ID: 38488637