A synthetic membrane shaper for controlled liposome deformation
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A synthetic membrane shaper for controlled liposome deformation. / Pezeshkian, Weria.
I: bioRxiv, 23.12.2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning
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TY - JOUR
T1 - A synthetic membrane shaper for controlled liposome deformation
AU - Pezeshkian, Weria
PY - 2021/12/23
Y1 - 2021/12/23
N2 - Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a ‘dumbbell’ shape, while organelles such as the autophagosome exhibit ‘stomatocyte’ shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call Synthetic Membrane Shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.
AB - Shape defines the structure and function of cellular membranes. In cell division, the cell membrane deforms into a ‘dumbbell’ shape, while organelles such as the autophagosome exhibit ‘stomatocyte’ shapes. Bottom-up in vitro reconstitution of protein machineries that stabilize or resolve the membrane necks in such deformed liposome structures is of considerable interest to characterize their function. Here we develop a DNA-nanotechnology-based approach that we call Synthetic Membrane Shaper (SMS), where cholesterol-linked DNA structures attach to the liposome membrane to reproducibly generate high yields of stomatocytes and dumbbells. In silico simulations confirm the shape-stabilizing role of the SMS. We show that the SMS is fully compatible with protein reconstitution by assembling bacterial divisome proteins (DynaminA, FtsZ:ZipA) at the catenoidal neck of these membrane structures. The SMS approach provides a general tool for studying protein binding to complex membrane geometries that will greatly benefit synthetic cell research.
U2 - 10.1101/2021.12.22.473854
DO - 10.1101/2021.12.22.473854
M3 - Journal article
JO - bioRxiv
JF - bioRxiv
SN - 2692-8205
ER -
ID: 316411656