A Representative Collection of Commensal Extended-Spectrum- and AmpC-β-Lactamase-Producing Escherichia coli of Animal Origin for Phage Sensitivity Studies
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Introduction: Extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase (AmpC)-producing Escherichia coli from livestock and meat represent a zoonotic risk and biocontrol solutions are needed to prevent transmission to humans. Methods: In this study, we established a representative collection of animal-origin ESBL/AmpC E. coli as target to test the antimicrobial potential of bacteriophages. Results: Bioinformatic analysis of whole-genome sequence data of 198 ESBL/AmpC E. coli from pigs, broilers, and broiler meat identified strains belonging to all known E. coli phylogroups and 65 multilocus sequence types. Various ESBL/AmpC genes and plasmid types were detected with expected source-specific patterns. Plaque assay using 15 phages previously isolated using the E. coli reference collection demonstrated that Warwickvirus phages showed the broadest host range, killing up to 26 strains. Conclusions:154/198 strains were resistant to infection by all phages tested, suggesting a need for isolating phages specific for ESBL/AmpC E. coli. The strain collection described in this study is a useful resource fulfilling such need.
Originalsprog | Engelsk |
---|---|
Tidsskrift | PHAGE: Therapy, Applications, and Research |
Vol/bind | 4 |
Udgave nummer | 1 |
Sider (fra-til) | 35-45 |
Antal sider | 11 |
ISSN | 2641-6530 |
DOI | |
Status | Udgivet - 2023 |
Bibliografisk note
Funding Information:
This project has received funding from Promilleafgiftsfonden, Denmark for A.R.V.
Publisher Copyright:
© 2023, Mary Ann Liebert, Inc., publishers.
ID: 343340376