A RECQ5-RNA polymerase II association identified by targeted proteomic analysis of human chromatin
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A RECQ5-RNA polymerase II association identified by targeted proteomic analysis of human chromatin. / Aygün, Ozan; Svejstrup, Jesper; Liu, Yilun.
I: Proceedings of the National Academy of Sciences of the United States of America, Bind 105, Nr. 25, 24.06.2008, s. 8580-8584.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A RECQ5-RNA polymerase II association identified by targeted proteomic analysis of human chromatin
AU - Aygün, Ozan
AU - Svejstrup, Jesper
AU - Liu, Yilun
PY - 2008/6/24
Y1 - 2008/6/24
N2 - Although the active forms of factors involved in DNA-related processes such as DNA replication, repair, and transcription are associated with chromatin, proteins are rarely purified from this source. Here, we describe a protocol for the isolation of chromatin-associated factors and use it to identify proteins interacting with human RNA polymerase II (RNAPII). Our data establish RECQ5 helicase as a bona fide RNAPII-associated protein. The RECQ5-RNAPII interaction is direct and is mediated by the RPB1 subunit of RNAPII, and RECQ5 appears to be the only member of the human RECQ family of helicases that associates with RNAPII. These data suggest an unexpected role for RECQ5 helicase at the interface of transcription and genomic stability.
AB - Although the active forms of factors involved in DNA-related processes such as DNA replication, repair, and transcription are associated with chromatin, proteins are rarely purified from this source. Here, we describe a protocol for the isolation of chromatin-associated factors and use it to identify proteins interacting with human RNA polymerase II (RNAPII). Our data establish RECQ5 helicase as a bona fide RNAPII-associated protein. The RECQ5-RNAPII interaction is direct and is mediated by the RPB1 subunit of RNAPII, and RECQ5 appears to be the only member of the human RECQ family of helicases that associates with RNAPII. These data suggest an unexpected role for RECQ5 helicase at the interface of transcription and genomic stability.
KW - BLM
KW - Genome integrity
KW - Transcription
KW - WRN
U2 - 10.1073/pnas.0804424105
DO - 10.1073/pnas.0804424105
M3 - Journal article
C2 - 18562274
AN - SCOPUS:47249100637
VL - 105
SP - 8580
EP - 8584
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 25
ER -
ID: 331006916