A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples. / Rossen, L.; Holmstrøm, Kim; Olsen, J. E.
I: International Journal of Food Microbiology, Bind 14, Nr. 2, 11.1991, s. 145-151.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples
AU - Rossen, L.
AU - Holmstrøm, Kim
AU - Olsen, J. E.
PY - 1991/11
Y1 - 1991/11
N2 - A rapid and simple assay has been developed which allows specific detection of Listeria monocytogenes within 3.5 h in cultures prepared from suspect food samples and propagated 48 h in selective medium. The assay is based on PCR technology, and uses a specific primer set derived from sequences located down-stream of the hlyA gene. The specificity of the primer set was confirmed by testing 115 L. monocytogenes, 14 L. innocua, 5 L. seeligeri and 4 L. ivanovii isolates. The assay was compared to standard microbiological tests and gave identical results for 83 food samples, including 32 positives. These field trials indicate that the assay developed provides an alternative detection system for L. monocytogenes in foods, which can be used by the food industry.
AB - A rapid and simple assay has been developed which allows specific detection of Listeria monocytogenes within 3.5 h in cultures prepared from suspect food samples and propagated 48 h in selective medium. The assay is based on PCR technology, and uses a specific primer set derived from sequences located down-stream of the hlyA gene. The specificity of the primer set was confirmed by testing 115 L. monocytogenes, 14 L. innocua, 5 L. seeligeri and 4 L. ivanovii isolates. The assay was compared to standard microbiological tests and gave identical results for 83 food samples, including 32 positives. These field trials indicate that the assay developed provides an alternative detection system for L. monocytogenes in foods, which can be used by the food industry.
KW - Detection
KW - DNA-probe
KW - Foods
KW - Listeria monocytogenes
UR - http://www.scopus.com/inward/record.url?scp=0025937803&partnerID=8YFLogxK
U2 - 10.1016/0168-1605(91)90101-T
DO - 10.1016/0168-1605(91)90101-T
M3 - Journal article
C2 - 1777383
AN - SCOPUS:0025937803
VL - 14
SP - 145
EP - 151
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
SN - 0168-1605
IS - 2
ER -
ID: 257697963