A gene regulatory network anchored by LIM homeobox 1 for embryonic head development
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
A gene regulatory network anchored by LIM homeobox 1 for embryonic head development. / Sibbritt, Tennille; Ip, Chi K.; Khoo, Poh Lynn; Wilkie, Emilie; Jones, Vanessa; Sun, Jane Q.J.; Shen, Joanne X.; Peng, Guangdun; Han, Jing Dong J.; Jing, Naihe; Osteil, Pierre; Ramialison, Mirana; Tam, Patrick P.L.; Fossat, Nicolas.
I: Genesis, Bind 56, Nr. 9, e23246, 2018.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - A gene regulatory network anchored by LIM homeobox 1 for embryonic head development
AU - Sibbritt, Tennille
AU - Ip, Chi K.
AU - Khoo, Poh Lynn
AU - Wilkie, Emilie
AU - Jones, Vanessa
AU - Sun, Jane Q.J.
AU - Shen, Joanne X.
AU - Peng, Guangdun
AU - Han, Jing Dong J.
AU - Jing, Naihe
AU - Osteil, Pierre
AU - Ramialison, Mirana
AU - Tam, Patrick P.L.
AU - Fossat, Nicolas
PY - 2018
Y1 - 2018
N2 - Development of the embryonic head is driven by the activity of gene regulatory networks of transcription factors. LHX1 is a homeobox transcription factor that plays an essential role in the formation of the embryonic head. The loss of LHX1 function results in anterior truncation of the embryo caused by the disruption of morphogenetic movement of tissue precursors and the dysregulation of WNT signaling activity. Profiling the gene expression pattern in the Lhx1 mutant embryo revealed that tissues in anterior germ layers acquire posterior tissue characteristics, suggesting LHX1 activity is required for the allocation and patterning of head precursor tissues. Here, we used LHX1 as an entry point to delineate its transcriptional targets and interactors and construct a LHX1-anchored gene regulatory network. Using a gain-of-function approach, we identified genes that immediately respond to Lhx1 activation. Meta-analysis of the datasets of LHX1-responsive genes and genes expressed in the anterior tissues of mouse embryos at head-fold stage, in conjunction with published Xenopus embryonic LHX1 (Xlim1) ChIP-seq data, has pinpointed the putative transcriptional targets of LHX1 and an array of genetic determinants functioning together in the formation of the mouse embryonic head.
AB - Development of the embryonic head is driven by the activity of gene regulatory networks of transcription factors. LHX1 is a homeobox transcription factor that plays an essential role in the formation of the embryonic head. The loss of LHX1 function results in anterior truncation of the embryo caused by the disruption of morphogenetic movement of tissue precursors and the dysregulation of WNT signaling activity. Profiling the gene expression pattern in the Lhx1 mutant embryo revealed that tissues in anterior germ layers acquire posterior tissue characteristics, suggesting LHX1 activity is required for the allocation and patterning of head precursor tissues. Here, we used LHX1 as an entry point to delineate its transcriptional targets and interactors and construct a LHX1-anchored gene regulatory network. Using a gain-of-function approach, we identified genes that immediately respond to Lhx1 activation. Meta-analysis of the datasets of LHX1-responsive genes and genes expressed in the anterior tissues of mouse embryos at head-fold stage, in conjunction with published Xenopus embryonic LHX1 (Xlim1) ChIP-seq data, has pinpointed the putative transcriptional targets of LHX1 and an array of genetic determinants functioning together in the formation of the mouse embryonic head.
KW - gene regulatory network
KW - Lhx1
KW - mouse
KW - WNT signaling
U2 - 10.1002/dvg.23246
DO - 10.1002/dvg.23246
M3 - Journal article
C2 - 30114334
AN - SCOPUS:85053481724
VL - 56
JO - Genesis: The Journal of Genetics and Development (Print)
JF - Genesis: The Journal of Genetics and Development (Print)
SN - 1526-954X
IS - 9
M1 - e23246
ER -
ID: 208877861