A clade of RHH proteins ubiquitous in Sulfolobales and their viruses regulates cell cycle progression
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A clade of RHH proteins ubiquitous in Sulfolobales and their viruses regulates cell cycle progression. / Li, Xuyang; Lozano-Madueno, Cristina; Martinez-Alvarez, Laura; Peng, Xu.
I: Nucleic acids symposium series, Bind 51, Nr. 4, 2023.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - A clade of RHH proteins ubiquitous in Sulfolobales and their viruses regulates cell cycle progression
AU - Li, Xuyang
AU - Lozano-Madueno, Cristina
AU - Martinez-Alvarez, Laura
AU - Peng, Xu
PY - 2023
Y1 - 2023
N2 - Cell cycle regulation is crucial for all living organisms and is often targeted by viruses to facilitate their own propagation, yet cell cycle progression control is largely underexplored in archaea. In this work, we reveal a cell cycle regulator (aCcr1) carrying a ribbon-helix-helix (RHH) domain and ubiquitous in the Thermoproteota of the order Sulfolobales and their viruses. Overexpression of several aCcr1 members including gp21 of rudivirus SIRV2 and its host homolog SiL_0190 of Saccharolobus islandicus LAL14/1 results in impairment of cell division, evidenced by growth retardation, cell enlargement and an increase in cellular DNA content. Additionally, both gp21 and SiL_0190 can bind to the motif AGTATTA conserved in the promoter of several genes involved in cell division, DNA replication and cellular metabolism thereby repressing or inducing their transcription. Our results suggest that aCcr1 silences cell division and drives progression to the S-phase in Sulfolobales, a function exploited by viruses to facilitate viral propagation.
AB - Cell cycle regulation is crucial for all living organisms and is often targeted by viruses to facilitate their own propagation, yet cell cycle progression control is largely underexplored in archaea. In this work, we reveal a cell cycle regulator (aCcr1) carrying a ribbon-helix-helix (RHH) domain and ubiquitous in the Thermoproteota of the order Sulfolobales and their viruses. Overexpression of several aCcr1 members including gp21 of rudivirus SIRV2 and its host homolog SiL_0190 of Saccharolobus islandicus LAL14/1 results in impairment of cell division, evidenced by growth retardation, cell enlargement and an increase in cellular DNA content. Additionally, both gp21 and SiL_0190 can bind to the motif AGTATTA conserved in the promoter of several genes involved in cell division, DNA replication and cellular metabolism thereby repressing or inducing their transcription. Our results suggest that aCcr1 silences cell division and drives progression to the S-phase in Sulfolobales, a function exploited by viruses to facilitate viral propagation.
KW - HYPERTHERMOPHILIC ARCHAEON
KW - CHROMOSOME SEGREGATION
KW - GENE-EXPRESSION
KW - DNA-BINDING
KW - DIVISION
KW - GENOME
KW - ALIGNMENT
KW - INSIGHTS
KW - PRODUCT
KW - DOMAIN
U2 - 10.1093/nar/gkad011
DO - 10.1093/nar/gkad011
M3 - Journal article
C2 - 36727447
VL - 51
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
SN - 0261-3166
IS - 4
ER -
ID: 336461768