TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF+ leukocytes

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Standard

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF+ leukocytes. / Reichwald, Kirsten; Jørgensen, Tina Z.; Tougaard, Peter; Skov, Søren.

In: PloS one, Vol. 9, No. 1, e85793, 2014.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Reichwald, K, Jørgensen, TZ, Tougaard, P & Skov, S 2014, 'TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF+ leukocytes', PloS one, vol. 9, no. 1, e85793. https://doi.org/10.1371/journal.pone.0085793

APA

Reichwald, K., Jørgensen, T. Z., Tougaard, P., & Skov, S. (2014). TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF+ leukocytes. PloS one, 9(1), [e85793]. https://doi.org/10.1371/journal.pone.0085793

Vancouver

Reichwald K, Jørgensen TZ, Tougaard P, Skov S. TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF+ leukocytes. PloS one. 2014;9(1). e85793. https://doi.org/10.1371/journal.pone.0085793

Author

Reichwald, Kirsten ; Jørgensen, Tina Z. ; Tougaard, Peter ; Skov, Søren. / TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF+ leukocytes. In: PloS one. 2014 ; Vol. 9, No. 1.

Bibtex

@article{4a0be22f66cd4d8c9eec635d1d2f18ad,
title = "TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF+ leukocytes",
abstract = "An elevated level of the cytokine TL1A is known to be associated with several autoimmune diseases, e.g. rheumatoid arthritis and inflammatory bowel disease. However, the mode of action of TL1A remains elusive. In this study, we investigated the role of TL1A in a pro-inflammatory setting, using human leukocytes purified from healthy donors. We show that TL1A, together with IL-12, IL-15 and IL-18, directly induces the production of IL-6 and TNF-α from leukocytes. Interestingly, TL1A-induced IL-6 was not produced by CD14(+) monocytes. We further show that the produced IL-6 is fully functional, as measured by its ability to signal through the IL-6 receptor, and that the induction of IL-6 is independent of TCR stimulation. Furthermore, the transcription factor PLZF was induced in stimulated cells. These results offer a substantial explanation for the role of TL1A, since TNF-α and IL-6 are directly responsible for much of the inflammatory state in many autoimmune diseases. Our study suggests that TL1A is a possible target for the treatment of autoimmune diseases.",
author = "Kirsten Reichwald and J{\o}rgensen, {Tina Z.} and Peter Tougaard and S{\o}ren Skov",
year = "2014",
doi = "10.1371/journal.pone.0085793",
language = "English",
volume = "9",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

RIS

TY - JOUR

T1 - TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF+ leukocytes

AU - Reichwald, Kirsten

AU - Jørgensen, Tina Z.

AU - Tougaard, Peter

AU - Skov, Søren

PY - 2014

Y1 - 2014

N2 - An elevated level of the cytokine TL1A is known to be associated with several autoimmune diseases, e.g. rheumatoid arthritis and inflammatory bowel disease. However, the mode of action of TL1A remains elusive. In this study, we investigated the role of TL1A in a pro-inflammatory setting, using human leukocytes purified from healthy donors. We show that TL1A, together with IL-12, IL-15 and IL-18, directly induces the production of IL-6 and TNF-α from leukocytes. Interestingly, TL1A-induced IL-6 was not produced by CD14(+) monocytes. We further show that the produced IL-6 is fully functional, as measured by its ability to signal through the IL-6 receptor, and that the induction of IL-6 is independent of TCR stimulation. Furthermore, the transcription factor PLZF was induced in stimulated cells. These results offer a substantial explanation for the role of TL1A, since TNF-α and IL-6 are directly responsible for much of the inflammatory state in many autoimmune diseases. Our study suggests that TL1A is a possible target for the treatment of autoimmune diseases.

AB - An elevated level of the cytokine TL1A is known to be associated with several autoimmune diseases, e.g. rheumatoid arthritis and inflammatory bowel disease. However, the mode of action of TL1A remains elusive. In this study, we investigated the role of TL1A in a pro-inflammatory setting, using human leukocytes purified from healthy donors. We show that TL1A, together with IL-12, IL-15 and IL-18, directly induces the production of IL-6 and TNF-α from leukocytes. Interestingly, TL1A-induced IL-6 was not produced by CD14(+) monocytes. We further show that the produced IL-6 is fully functional, as measured by its ability to signal through the IL-6 receptor, and that the induction of IL-6 is independent of TCR stimulation. Furthermore, the transcription factor PLZF was induced in stimulated cells. These results offer a substantial explanation for the role of TL1A, since TNF-α and IL-6 are directly responsible for much of the inflammatory state in many autoimmune diseases. Our study suggests that TL1A is a possible target for the treatment of autoimmune diseases.

U2 - 10.1371/journal.pone.0085793

DO - 10.1371/journal.pone.0085793

M3 - Journal article

C2 - 24416448

VL - 9

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 1

M1 - e85793

ER -

ID: 100624880