Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. / Hansen, Line; Larsen, Esben Kjær Unmack; Nielsen, Erik Holm; Iversen, Frank Horsbøl; Liu, Zhuo; Thomsen, Karen Sand; Pedersen, Michael; Skrydstrup, Troels; Nielsen, Niels Chr; Ploug, Michael; Kjems, Jørgen.

In: Nanoscale, Vol. 5, No. 17, 07.09.2013, p. 8192-201.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, L, Larsen, EKU, Nielsen, EH, Iversen, FH, Liu, Z, Thomsen, KS, Pedersen, M, Skrydstrup, T, Nielsen, NC, Ploug, M & Kjems, J 2013, 'Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells', Nanoscale, vol. 5, no. 17, pp. 8192-201. https://doi.org/10.1039/c3nr32922d

APA

Hansen, L., Larsen, E. K. U., Nielsen, E. H., Iversen, F. H., Liu, Z., Thomsen, K. S., Pedersen, M., Skrydstrup, T., Nielsen, N. C., Ploug, M., & Kjems, J. (2013). Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. Nanoscale, 5(17), 8192-201. https://doi.org/10.1039/c3nr32922d

Vancouver

Hansen L, Larsen EKU, Nielsen EH, Iversen FH, Liu Z, Thomsen KS et al. Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. Nanoscale. 2013 Sep 7;5(17):8192-201. https://doi.org/10.1039/c3nr32922d

Author

Hansen, Line ; Larsen, Esben Kjær Unmack ; Nielsen, Erik Holm ; Iversen, Frank Horsbøl ; Liu, Zhuo ; Thomsen, Karen Sand ; Pedersen, Michael ; Skrydstrup, Troels ; Nielsen, Niels Chr ; Ploug, Michael ; Kjems, Jørgen. / Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells. In: Nanoscale. 2013 ; Vol. 5, No. 17. pp. 8192-201.

Bibtex

@article{a69f98009ee94877a4ca244665f6ee24,
title = "Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells",
abstract = "Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient management when combined with MRI and drug delivery.",
keywords = "Amino Acid Sequence, Click Chemistry, Ferric Compounds, Fluorescent Dyes, HEK293 Cells, Humans, Magnetite Nanoparticles, Microscopy, Confocal, Peptides, Polyethylene Glycols, Protein Binding, Receptors, Urokinase Plasminogen Activator",
author = "Line Hansen and Larsen, {Esben Kj{\ae}r Unmack} and Nielsen, {Erik Holm} and Iversen, {Frank Horsb{\o}l} and Zhuo Liu and Thomsen, {Karen Sand} and Michael Pedersen and Troels Skrydstrup and Nielsen, {Niels Chr} and Michael Ploug and J{\o}rgen Kjems",
year = "2013",
month = sep,
day = "7",
doi = "10.1039/c3nr32922d",
language = "English",
volume = "5",
pages = "8192--201",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "Royal Society of Chemistry",
number = "17",

}

RIS

TY - JOUR

T1 - Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells

AU - Hansen, Line

AU - Larsen, Esben Kjær Unmack

AU - Nielsen, Erik Holm

AU - Iversen, Frank Horsbøl

AU - Liu, Zhuo

AU - Thomsen, Karen Sand

AU - Pedersen, Michael

AU - Skrydstrup, Troels

AU - Nielsen, Niels Chr

AU - Ploug, Michael

AU - Kjems, Jørgen

PY - 2013/9/7

Y1 - 2013/9/7

N2 - Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient management when combined with MRI and drug delivery.

AB - Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient management when combined with MRI and drug delivery.

KW - Amino Acid Sequence

KW - Click Chemistry

KW - Ferric Compounds

KW - Fluorescent Dyes

KW - HEK293 Cells

KW - Humans

KW - Magnetite Nanoparticles

KW - Microscopy, Confocal

KW - Peptides

KW - Polyethylene Glycols

KW - Protein Binding

KW - Receptors, Urokinase Plasminogen Activator

U2 - 10.1039/c3nr32922d

DO - 10.1039/c3nr32922d

M3 - Journal article

C2 - 23835641

VL - 5

SP - 8192

EP - 8201

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 17

ER -

ID: 107124749