Rac1-dependent recruitment of PAK2 to G 2 phase centrosomes and their roles in the regulation of mitotic entry

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Rac1-dependent recruitment of PAK2 to G 2 phase centrosomes and their roles in the regulation of mitotic entry. / May, Martin; Schelle, Ilona; Brakebusch, Cord Herbert; Rottner, Klemens; Genth, Harald.

In: Cell Cycle, Vol. 13, No. 14, 19.05.2014, p. 2211-21.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

May, M, Schelle, I, Brakebusch, CH, Rottner, K & Genth, H 2014, 'Rac1-dependent recruitment of PAK2 to G 2 phase centrosomes and their roles in the regulation of mitotic entry', Cell Cycle, vol. 13, no. 14, pp. 2211-21. https://doi.org/10.4161/cc.29279

APA

May, M., Schelle, I., Brakebusch, C. H., Rottner, K., & Genth, H. (2014). Rac1-dependent recruitment of PAK2 to G 2 phase centrosomes and their roles in the regulation of mitotic entry. Cell Cycle, 13(14), 2211-21. https://doi.org/10.4161/cc.29279

Vancouver

May M, Schelle I, Brakebusch CH, Rottner K, Genth H. Rac1-dependent recruitment of PAK2 to G 2 phase centrosomes and their roles in the regulation of mitotic entry. Cell Cycle. 2014 May 19;13(14):2211-21. https://doi.org/10.4161/cc.29279

Author

May, Martin ; Schelle, Ilona ; Brakebusch, Cord Herbert ; Rottner, Klemens ; Genth, Harald. / Rac1-dependent recruitment of PAK2 to G 2 phase centrosomes and their roles in the regulation of mitotic entry. In: Cell Cycle. 2014 ; Vol. 13, No. 14. pp. 2211-21.

Bibtex

@article{548c927243744d2e9eabcf6a9edbb42d,
title = "Rac1-dependent recruitment of PAK2 to G 2 phase centrosomes and their roles in the regulation of mitotic entry",
abstract = "During mitotic entry, the centrosomes provide a scaffold for initial activation of the CyclinB/Cdk1 complex, the mitotic kinase Aurora A, and the Aurora A-activating kinase p21-activated kinase (PAK). The activation of PAK at the centrosomes is yet regarded to happen independently of the Rho-GTPases Rac/Cdc42. In this study, Rac1 (but not RhoA or Cdc42) is presented to associate with the centrosomes from early G 2 phase until prometaphase in a cell cycle-dependent fashion, as evidenced by western blot analysis of prepared centrosomes and by immunolabeling. PAK associates with the G 2/M-phase centrosomes in a Rac1-dependent fashion. Furthermore, specific inhibition of Rac1 by C. difficile toxinB-catalyzed glucosylation or by knockout results in inhibited activation of PAK1/2, Aurora A, and the CyclinB/Cdk1 complex in late G 2 phase/prophase and delayed mitotic entry. Inhibition of PAK activation at late G 2-phase centrosomes caused by Rac1 inactivation coincides with impeded activation of Aurora A and the CyclinB/Cdk1 complex and delayed mitotic entry.",
author = "Martin May and Ilona Schelle and Brakebusch, {Cord Herbert} and Klemens Rottner and Harald Genth",
year = "2014",
month = may,
day = "19",
doi = "10.4161/cc.29279",
language = "English",
volume = "13",
pages = "2211--21",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Taylor & Francis",
number = "14",

}

RIS

TY - JOUR

T1 - Rac1-dependent recruitment of PAK2 to G 2 phase centrosomes and their roles in the regulation of mitotic entry

AU - May, Martin

AU - Schelle, Ilona

AU - Brakebusch, Cord Herbert

AU - Rottner, Klemens

AU - Genth, Harald

PY - 2014/5/19

Y1 - 2014/5/19

N2 - During mitotic entry, the centrosomes provide a scaffold for initial activation of the CyclinB/Cdk1 complex, the mitotic kinase Aurora A, and the Aurora A-activating kinase p21-activated kinase (PAK). The activation of PAK at the centrosomes is yet regarded to happen independently of the Rho-GTPases Rac/Cdc42. In this study, Rac1 (but not RhoA or Cdc42) is presented to associate with the centrosomes from early G 2 phase until prometaphase in a cell cycle-dependent fashion, as evidenced by western blot analysis of prepared centrosomes and by immunolabeling. PAK associates with the G 2/M-phase centrosomes in a Rac1-dependent fashion. Furthermore, specific inhibition of Rac1 by C. difficile toxinB-catalyzed glucosylation or by knockout results in inhibited activation of PAK1/2, Aurora A, and the CyclinB/Cdk1 complex in late G 2 phase/prophase and delayed mitotic entry. Inhibition of PAK activation at late G 2-phase centrosomes caused by Rac1 inactivation coincides with impeded activation of Aurora A and the CyclinB/Cdk1 complex and delayed mitotic entry.

AB - During mitotic entry, the centrosomes provide a scaffold for initial activation of the CyclinB/Cdk1 complex, the mitotic kinase Aurora A, and the Aurora A-activating kinase p21-activated kinase (PAK). The activation of PAK at the centrosomes is yet regarded to happen independently of the Rho-GTPases Rac/Cdc42. In this study, Rac1 (but not RhoA or Cdc42) is presented to associate with the centrosomes from early G 2 phase until prometaphase in a cell cycle-dependent fashion, as evidenced by western blot analysis of prepared centrosomes and by immunolabeling. PAK associates with the G 2/M-phase centrosomes in a Rac1-dependent fashion. Furthermore, specific inhibition of Rac1 by C. difficile toxinB-catalyzed glucosylation or by knockout results in inhibited activation of PAK1/2, Aurora A, and the CyclinB/Cdk1 complex in late G 2 phase/prophase and delayed mitotic entry. Inhibition of PAK activation at late G 2-phase centrosomes caused by Rac1 inactivation coincides with impeded activation of Aurora A and the CyclinB/Cdk1 complex and delayed mitotic entry.

U2 - 10.4161/cc.29279

DO - 10.4161/cc.29279

M3 - Journal article

C2 - 24840740

VL - 13

SP - 2211

EP - 2221

JO - Cell Cycle

JF - Cell Cycle

SN - 1538-4101

IS - 14

ER -

ID: 112840657