Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice
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Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. / Serup, Palle; Gustavsen, Carsten; Klein, Tino; Potter, Leah A; Lin, Robert; Mullapudi, Nandita; Wandzioch, Ewa; Hines, Angela; Davis, Ashley; Bruun, Christine; Engberg, Nina; Petersen, Dorthe R; Peterslund, Janny M L; Macdonald, Raymond J; Grapin-Botton, Anne; Magnuson, Mark A; Zaret, Kenneth S.
In: Disease Models and Mechanisms, Vol. 5, No. 6, 01.11.2012, p. 956-66.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice
AU - Serup, Palle
AU - Gustavsen, Carsten
AU - Klein, Tino
AU - Potter, Leah A
AU - Lin, Robert
AU - Mullapudi, Nandita
AU - Wandzioch, Ewa
AU - Hines, Angela
AU - Davis, Ashley
AU - Bruun, Christine
AU - Engberg, Nina
AU - Petersen, Dorthe R
AU - Peterslund, Janny M L
AU - Macdonald, Raymond J
AU - Grapin-Botton, Anne
AU - Magnuson, Mark A
AU - Zaret, Kenneth S
PY - 2012/11/1
Y1 - 2012/11/1
N2 - Extracellular signals in development, physiology, homeostasis and disease often act by regulating transcription. Herein we describe a general method and specific resources for determining where and when such signaling occurs in live animals and for systematically comparing the timing and extent of different signals in different cellular contexts. We used recombinase-mediated cassette exchange (RMCE) to test the effect of successively deleting conserved genomic regions of the ubiquitously active Rosa26 promoter and substituting the deleted regions for regulatory sequences that respond to diverse extracellular signals. We thereby created an allelic series of embryonic stem cells and mice, each containing a signal-responsive sentinel with different fluorescent reporters that respond with sensitivity and specificity to retinoic acids, bone morphogenic proteins, activin A, Wnts or Notch, and that can be adapted to any pathway that acts via DNA elements.
AB - Extracellular signals in development, physiology, homeostasis and disease often act by regulating transcription. Herein we describe a general method and specific resources for determining where and when such signaling occurs in live animals and for systematically comparing the timing and extent of different signals in different cellular contexts. We used recombinase-mediated cassette exchange (RMCE) to test the effect of successively deleting conserved genomic regions of the ubiquitously active Rosa26 promoter and substituting the deleted regions for regulatory sequences that respond to diverse extracellular signals. We thereby created an allelic series of embryonic stem cells and mice, each containing a signal-responsive sentinel with different fluorescent reporters that respond with sensitivity and specificity to retinoic acids, bone morphogenic proteins, activin A, Wnts or Notch, and that can be adapted to any pathway that acts via DNA elements.
U2 - 10.1242/dmm.009696
DO - 10.1242/dmm.009696
M3 - Journal article
C2 - 22888097
VL - 5
SP - 956
EP - 966
JO - Disease Models & Mechanisms
JF - Disease Models & Mechanisms
SN - 1754-8403
IS - 6
ER -
ID: 42004992