Monoclonal antibodies for the detection of Puccinia striiformis urediniospores

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Monoclonal antibodies for the detection of Puccinia striiformis urediniospores. / Skottrup, Peter Durand; Frøkiær, Hanne; Hearty, Stephen; O'Kennedy, Richard; Hejgaard, Jørn; Nicolaisen, Mogens; Justesen, Annemarie Fejer.

In: Mycological Research, Vol. 111, 2007, p. 332-338.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Skottrup, PD, Frøkiær, H, Hearty, S, O'Kennedy, R, Hejgaard, J, Nicolaisen, M & Justesen, AF 2007, 'Monoclonal antibodies for the detection of Puccinia striiformis urediniospores', Mycological Research, vol. 111, pp. 332-338.

APA

Skottrup, P. D., Frøkiær, H., Hearty, S., O'Kennedy, R., Hejgaard, J., Nicolaisen, M., & Justesen, A. F. (2007). Monoclonal antibodies for the detection of Puccinia striiformis urediniospores. Mycological Research, 111, 332-338.

Vancouver

Skottrup PD, Frøkiær H, Hearty S, O'Kennedy R, Hejgaard J, Nicolaisen M et al. Monoclonal antibodies for the detection of Puccinia striiformis urediniospores. Mycological Research. 2007;111:332-338.

Author

Skottrup, Peter Durand ; Frøkiær, Hanne ; Hearty, Stephen ; O'Kennedy, Richard ; Hejgaard, Jørn ; Nicolaisen, Mogens ; Justesen, Annemarie Fejer. / Monoclonal antibodies for the detection of Puccinia striiformis urediniospores. In: Mycological Research. 2007 ; Vol. 111. pp. 332-338.

Bibtex

@article{e11e19318e6e4ef584a68a11ab1b816e,
title = "Monoclonal antibodies for the detection of Puccinia striiformis urediniospores",
abstract = "The fungal pathogen Pst causes yellow rust disease in wheat plants leading to crop losses. The organism spreads by releasing wind-dispersed urediniospores from infected plants. In this study a library of novel monoclonal antibodies (mAbs) was developed against Pst urediniospores. Nine mAb-producing cell lines were cloned and their cross-reactivities characterised against a panel of airborne fungal spores representing genera commonly found in the same environment as Pst. Two specific mAbs were used to develop a competitive ELISA (Pst mAb4) and a subtractive inhibition ELISA (Pst mAb8). Standard curves for both assays had good intra- and interday reproducibility. The subtractive inhibition ELISA had greater sensitivity with a detection limit of 1.5 105 spores ml1. Cross-reactivity studies of Pst mAb8 in the subtractive inhibition ELISA, showed reaction with other Puccinia spores only, suggesting that common epitopes exist within this genus. The biosensor-compatible Pst mAb8 assay principle developed in this study has the potential to be implemented in future {\^a}¿¿label-free{\^a}¿¿ in-the-field systems for Pst detection.",
author = "Skottrup, {Peter Durand} and Hanne Fr{\o}ki{\ae}r and Stephen Hearty and Richard O'Kennedy and J{\o}rn Hejgaard and Mogens Nicolaisen and Justesen, {Annemarie Fejer}",
year = "2007",
language = "English",
volume = "111",
pages = "332--338",
journal = "Fungal Biology",
issn = "1878-6146",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Monoclonal antibodies for the detection of Puccinia striiformis urediniospores

AU - Skottrup, Peter Durand

AU - Frøkiær, Hanne

AU - Hearty, Stephen

AU - O'Kennedy, Richard

AU - Hejgaard, Jørn

AU - Nicolaisen, Mogens

AU - Justesen, Annemarie Fejer

PY - 2007

Y1 - 2007

N2 - The fungal pathogen Pst causes yellow rust disease in wheat plants leading to crop losses. The organism spreads by releasing wind-dispersed urediniospores from infected plants. In this study a library of novel monoclonal antibodies (mAbs) was developed against Pst urediniospores. Nine mAb-producing cell lines were cloned and their cross-reactivities characterised against a panel of airborne fungal spores representing genera commonly found in the same environment as Pst. Two specific mAbs were used to develop a competitive ELISA (Pst mAb4) and a subtractive inhibition ELISA (Pst mAb8). Standard curves for both assays had good intra- and interday reproducibility. The subtractive inhibition ELISA had greater sensitivity with a detection limit of 1.5 105 spores ml1. Cross-reactivity studies of Pst mAb8 in the subtractive inhibition ELISA, showed reaction with other Puccinia spores only, suggesting that common epitopes exist within this genus. The biosensor-compatible Pst mAb8 assay principle developed in this study has the potential to be implemented in future ⿿label-free⿿ in-the-field systems for Pst detection.

AB - The fungal pathogen Pst causes yellow rust disease in wheat plants leading to crop losses. The organism spreads by releasing wind-dispersed urediniospores from infected plants. In this study a library of novel monoclonal antibodies (mAbs) was developed against Pst urediniospores. Nine mAb-producing cell lines were cloned and their cross-reactivities characterised against a panel of airborne fungal spores representing genera commonly found in the same environment as Pst. Two specific mAbs were used to develop a competitive ELISA (Pst mAb4) and a subtractive inhibition ELISA (Pst mAb8). Standard curves for both assays had good intra- and interday reproducibility. The subtractive inhibition ELISA had greater sensitivity with a detection limit of 1.5 105 spores ml1. Cross-reactivity studies of Pst mAb8 in the subtractive inhibition ELISA, showed reaction with other Puccinia spores only, suggesting that common epitopes exist within this genus. The biosensor-compatible Pst mAb8 assay principle developed in this study has the potential to be implemented in future ⿿label-free⿿ in-the-field systems for Pst detection.

M3 - Journal article

VL - 111

SP - 332

EP - 338

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

ER -

ID: 33226422