M2-like macrophages are responsible for collagen degradation through a mannose receptor-mediated pathway
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M2-like macrophages are responsible for collagen degradation through a mannose receptor-mediated pathway. / Madsen, Daniel H; Leonard, Daniel; Masedunskas, Andrius; Moyer, Amanda; Jürgensen, Henrik Jessen; Peters, Diane E; Amornphimoltham, Panomwat; Selvaraj, Arul; Yamada, Susan S; Brenner, David A; Burgdorf, Sven; Engelholm, Lars H; Behrendt, Niels; Holmbeck, Kenn; Weigert, Roberto; Bugge, Thomas H.
In: Journal of Cell Biology, Vol. 202, No. 6, 16.09.2013, p. 951-66.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - M2-like macrophages are responsible for collagen degradation through a mannose receptor-mediated pathway
AU - Madsen, Daniel H
AU - Leonard, Daniel
AU - Masedunskas, Andrius
AU - Moyer, Amanda
AU - Jürgensen, Henrik Jessen
AU - Peters, Diane E
AU - Amornphimoltham, Panomwat
AU - Selvaraj, Arul
AU - Yamada, Susan S
AU - Brenner, David A
AU - Burgdorf, Sven
AU - Engelholm, Lars H
AU - Behrendt, Niels
AU - Holmbeck, Kenn
AU - Weigert, Roberto
AU - Bugge, Thomas H
PY - 2013/9/16
Y1 - 2013/9/16
N2 - Tissue remodeling processes critically depend on the timely removal and remodeling of preexisting collagen scaffolds. Nevertheless, many aspects related to the turnover of this abundant extracellular matrix component in vivo are still incompletely understood. We therefore took advantage of recent advances in optical imaging to develop an assay to visualize collagen turnover in situ and identify cell types and molecules involved in this process. Collagen introduced into the dermis of mice underwent cellular endocytosis in a partially matrix metalloproteinase-dependent manner and was subsequently routed to lysosomes for complete degradation. Collagen uptake was predominantly executed by a quantitatively minor population of M2-like macrophages, whereas more abundant Col1a1-expressing fibroblasts and Cx3cr1-expressing macrophages internalized collagen at lower levels. Genetic ablation of the collagen receptors mannose receptor (Mrc1) and urokinase plasminogen activator receptor-associated protein (Endo180 and Mrc2) impaired this intracellular collagen degradation pathway. This study demonstrates the importance of receptor-mediated cellular uptake to collagen turnover in vivo and identifies a key role of M2-like macrophages in this process.
AB - Tissue remodeling processes critically depend on the timely removal and remodeling of preexisting collagen scaffolds. Nevertheless, many aspects related to the turnover of this abundant extracellular matrix component in vivo are still incompletely understood. We therefore took advantage of recent advances in optical imaging to develop an assay to visualize collagen turnover in situ and identify cell types and molecules involved in this process. Collagen introduced into the dermis of mice underwent cellular endocytosis in a partially matrix metalloproteinase-dependent manner and was subsequently routed to lysosomes for complete degradation. Collagen uptake was predominantly executed by a quantitatively minor population of M2-like macrophages, whereas more abundant Col1a1-expressing fibroblasts and Cx3cr1-expressing macrophages internalized collagen at lower levels. Genetic ablation of the collagen receptors mannose receptor (Mrc1) and urokinase plasminogen activator receptor-associated protein (Endo180 and Mrc2) impaired this intracellular collagen degradation pathway. This study demonstrates the importance of receptor-mediated cellular uptake to collagen turnover in vivo and identifies a key role of M2-like macrophages in this process.
KW - Animals
KW - Apoptosis
KW - Blotting, Western
KW - Cell Proliferation
KW - Cells, Cultured
KW - Collagen
KW - Collagen Type I
KW - Endocytosis
KW - Female
KW - Fibroblasts
KW - Green Fluorescent Proteins
KW - Humans
KW - Immunoenzyme Techniques
KW - Lysosomes
KW - Macrophages
KW - Membrane Glycoproteins
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Knockout
KW - Mice, Transgenic
KW - RNA, Messenger
KW - Real-Time Polymerase Chain Reaction
KW - Receptors, Cell Surface
KW - Receptors, Chemokine
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Signal Transduction
U2 - 10.1083/jcb.201301081
DO - 10.1083/jcb.201301081
M3 - Journal article
C2 - 24019537
VL - 202
SP - 951
EP - 966
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 6
ER -
ID: 107122509