Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition. / Mouritsen, Annette; Søeborg, Tue; Johannsen, Trine Holm; Aksglaede, Lise; Sørensen, Kaspar; Hagen, Casper P; Mieritz, Mikkel G; Frederiksen, Hanne; Andersson, Anna-Maria; Juul, Anders.

In: Hormone Research in Paediatrics, Vol. 82, No. 1, 2014, p. 12-17.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mouritsen, A, Søeborg, T, Johannsen, TH, Aksglaede, L, Sørensen, K, Hagen, CP, Mieritz, MG, Frederiksen, H, Andersson, A-M & Juul, A 2014, 'Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition', Hormone Research in Paediatrics, vol. 82, no. 1, pp. 12-17. https://doi.org/10.1159/000358560

APA

Mouritsen, A., Søeborg, T., Johannsen, T. H., Aksglaede, L., Sørensen, K., Hagen, C. P., Mieritz, M. G., Frederiksen, H., Andersson, A-M., & Juul, A. (2014). Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition. Hormone Research in Paediatrics, 82(1), 12-17. https://doi.org/10.1159/000358560

Vancouver

Mouritsen A, Søeborg T, Johannsen TH, Aksglaede L, Sørensen K, Hagen CP et al. Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition. Hormone Research in Paediatrics. 2014;82(1):12-17. https://doi.org/10.1159/000358560

Author

Mouritsen, Annette ; Søeborg, Tue ; Johannsen, Trine Holm ; Aksglaede, Lise ; Sørensen, Kaspar ; Hagen, Casper P ; Mieritz, Mikkel G ; Frederiksen, Hanne ; Andersson, Anna-Maria ; Juul, Anders. / Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition. In: Hormone Research in Paediatrics. 2014 ; Vol. 82, No. 1. pp. 12-17.

Bibtex

@article{9942ec4c354c4393a5e22d655a4de911,
title = "Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition",
abstract = "BACKGROUND: Accurate and selective assessment of testosterone requires use of a sensitive LC-MS/MS method, especially at low levels as those seen in young children.METHODS: The present longitudinal study of 20 healthy children from the Copenhagen Puberty Study followed every 6 months for 5 years evaluates the longitudinal increase in serum testosterone before, during and after pubertal onset quantified by a newly developed LC-MS/MS method in comparison with immunoassay. Testosterone concentrations in serum samples (n = 177) were determined by LC-MS/MS (detection limit 0.1 nmol/l) and by immunoassay (detection limit 0.23 nmol/l).RESULTS: Serum concentrations of testosterone increased gradually with age by both methods. However, serum testosterone was quantifiable in 9/10 girls prior to pubic hair development measured with LC-MS/MS, and in 2/10 girls measured with immunoassay. In boys, testosterone was quantifiable in 10/10 boys 1 year prior to pubic hair development measured with LC-MS/MS, and only in 1/10 boys measured with immunoassay. Serum testosterone levels were quantifiable 1.5 years (range 0.5-2.5) earlier using LC-MS/MS.CONCLUSION: Assessment of longitudinal circulating levels of serum testosterone using a selective LC-MS/MS method proved to be more sensitive in predicting early peripubertal changes in healthy children compared to levels determined by immunoassay.",
keywords = "Adolescent, Aging, Child, Female, Follow-Up Studies, Humans, Male, Mass Spectrometry, Puberty, Radioimmunoassay, Sex Characteristics, Testosterone",
author = "Annette Mouritsen and Tue S{\o}eborg and Johannsen, {Trine Holm} and Lise Aksglaede and Kaspar S{\o}rensen and Hagen, {Casper P} and Mieritz, {Mikkel G} and Hanne Frederiksen and Anna-Maria Andersson and Anders Juul",
year = "2014",
doi = "10.1159/000358560",
language = "English",
volume = "82",
pages = "12--17",
journal = "Hormone Research in Paediatrics",
issn = "1663-2818",
publisher = "S Karger AG",
number = "1",

}

RIS

TY - JOUR

T1 - Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition

AU - Mouritsen, Annette

AU - Søeborg, Tue

AU - Johannsen, Trine Holm

AU - Aksglaede, Lise

AU - Sørensen, Kaspar

AU - Hagen, Casper P

AU - Mieritz, Mikkel G

AU - Frederiksen, Hanne

AU - Andersson, Anna-Maria

AU - Juul, Anders

PY - 2014

Y1 - 2014

N2 - BACKGROUND: Accurate and selective assessment of testosterone requires use of a sensitive LC-MS/MS method, especially at low levels as those seen in young children.METHODS: The present longitudinal study of 20 healthy children from the Copenhagen Puberty Study followed every 6 months for 5 years evaluates the longitudinal increase in serum testosterone before, during and after pubertal onset quantified by a newly developed LC-MS/MS method in comparison with immunoassay. Testosterone concentrations in serum samples (n = 177) were determined by LC-MS/MS (detection limit 0.1 nmol/l) and by immunoassay (detection limit 0.23 nmol/l).RESULTS: Serum concentrations of testosterone increased gradually with age by both methods. However, serum testosterone was quantifiable in 9/10 girls prior to pubic hair development measured with LC-MS/MS, and in 2/10 girls measured with immunoassay. In boys, testosterone was quantifiable in 10/10 boys 1 year prior to pubic hair development measured with LC-MS/MS, and only in 1/10 boys measured with immunoassay. Serum testosterone levels were quantifiable 1.5 years (range 0.5-2.5) earlier using LC-MS/MS.CONCLUSION: Assessment of longitudinal circulating levels of serum testosterone using a selective LC-MS/MS method proved to be more sensitive in predicting early peripubertal changes in healthy children compared to levels determined by immunoassay.

AB - BACKGROUND: Accurate and selective assessment of testosterone requires use of a sensitive LC-MS/MS method, especially at low levels as those seen in young children.METHODS: The present longitudinal study of 20 healthy children from the Copenhagen Puberty Study followed every 6 months for 5 years evaluates the longitudinal increase in serum testosterone before, during and after pubertal onset quantified by a newly developed LC-MS/MS method in comparison with immunoassay. Testosterone concentrations in serum samples (n = 177) were determined by LC-MS/MS (detection limit 0.1 nmol/l) and by immunoassay (detection limit 0.23 nmol/l).RESULTS: Serum concentrations of testosterone increased gradually with age by both methods. However, serum testosterone was quantifiable in 9/10 girls prior to pubic hair development measured with LC-MS/MS, and in 2/10 girls measured with immunoassay. In boys, testosterone was quantifiable in 10/10 boys 1 year prior to pubic hair development measured with LC-MS/MS, and only in 1/10 boys measured with immunoassay. Serum testosterone levels were quantifiable 1.5 years (range 0.5-2.5) earlier using LC-MS/MS.CONCLUSION: Assessment of longitudinal circulating levels of serum testosterone using a selective LC-MS/MS method proved to be more sensitive in predicting early peripubertal changes in healthy children compared to levels determined by immunoassay.

KW - Adolescent

KW - Aging

KW - Child

KW - Female

KW - Follow-Up Studies

KW - Humans

KW - Male

KW - Mass Spectrometry

KW - Puberty

KW - Radioimmunoassay

KW - Sex Characteristics

KW - Testosterone

U2 - 10.1159/000358560

DO - 10.1159/000358560

M3 - Journal article

C2 - 25033974

VL - 82

SP - 12

EP - 17

JO - Hormone Research in Paediatrics

JF - Hormone Research in Paediatrics

SN - 1663-2818

IS - 1

ER -

ID: 138130211