Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction: basic guidelines

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction : basic guidelines. / Parhamifar, Ladan; Wu, LinPing; Andersen, Helene; Moghimi, S Moein.

In: Methods, Vol. 68, No. 2, 01.07.2014, p. 300-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Parhamifar, L, Wu, L, Andersen, H & Moghimi, SM 2014, 'Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction: basic guidelines', Methods, vol. 68, no. 2, pp. 300-7. https://doi.org/10.1016/j.ymeth.2014.02.004

APA

Parhamifar, L., Wu, L., Andersen, H., & Moghimi, S. M. (2014). Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction: basic guidelines. Methods, 68(2), 300-7. https://doi.org/10.1016/j.ymeth.2014.02.004

Vancouver

Parhamifar L, Wu L, Andersen H, Moghimi SM. Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction: basic guidelines. Methods. 2014 Jul 1;68(2):300-7. https://doi.org/10.1016/j.ymeth.2014.02.004

Author

Parhamifar, Ladan ; Wu, LinPing ; Andersen, Helene ; Moghimi, S Moein. / Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction : basic guidelines. In: Methods. 2014 ; Vol. 68, No. 2. pp. 300-7.

Bibtex

@article{04019677e5c24849b3a3ada1cc406e78,
title = "Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction: basic guidelines",
abstract = "A myriad of cationic polymeric delivery vehicles are currently being developed with the aim of transporting various forms of nucleic acids to mammalian cells. The complexes between polycations and nucleic acids are referred to as polyplexes. The screening for successful polyplex candidates requires interdisciplinary research platforms and techniques for a more profound understanding of biophysical properties of delivery vehicles and their biological performance, including stability, transfection efficacy and possible cytotoxicity. Fluorescent microscopy has proven to be a useful tool for real-time monitoring of performance and intracellular trafficking of polyplexes as well as for assessing cell functionality. This review highlights the application of some of the most promising fluorescent microscopy platforms in relation to polyplex-mediated transfection processes.",
author = "Ladan Parhamifar and LinPing Wu and Helene Andersen and Moghimi, {S Moein}",
note = "Copyright {\textcopyright} 2014 Elsevier Inc. All rights reserved.",
year = "2014",
month = jul,
day = "1",
doi = "10.1016/j.ymeth.2014.02.004",
language = "English",
volume = "68",
pages = "300--7",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction

T2 - basic guidelines

AU - Parhamifar, Ladan

AU - Wu, LinPing

AU - Andersen, Helene

AU - Moghimi, S Moein

N1 - Copyright © 2014 Elsevier Inc. All rights reserved.

PY - 2014/7/1

Y1 - 2014/7/1

N2 - A myriad of cationic polymeric delivery vehicles are currently being developed with the aim of transporting various forms of nucleic acids to mammalian cells. The complexes between polycations and nucleic acids are referred to as polyplexes. The screening for successful polyplex candidates requires interdisciplinary research platforms and techniques for a more profound understanding of biophysical properties of delivery vehicles and their biological performance, including stability, transfection efficacy and possible cytotoxicity. Fluorescent microscopy has proven to be a useful tool for real-time monitoring of performance and intracellular trafficking of polyplexes as well as for assessing cell functionality. This review highlights the application of some of the most promising fluorescent microscopy platforms in relation to polyplex-mediated transfection processes.

AB - A myriad of cationic polymeric delivery vehicles are currently being developed with the aim of transporting various forms of nucleic acids to mammalian cells. The complexes between polycations and nucleic acids are referred to as polyplexes. The screening for successful polyplex candidates requires interdisciplinary research platforms and techniques for a more profound understanding of biophysical properties of delivery vehicles and their biological performance, including stability, transfection efficacy and possible cytotoxicity. Fluorescent microscopy has proven to be a useful tool for real-time monitoring of performance and intracellular trafficking of polyplexes as well as for assessing cell functionality. This review highlights the application of some of the most promising fluorescent microscopy platforms in relation to polyplex-mediated transfection processes.

U2 - 10.1016/j.ymeth.2014.02.004

DO - 10.1016/j.ymeth.2014.02.004

M3 - Journal article

C2 - 24561166

VL - 68

SP - 300

EP - 307

JO - Methods

JF - Methods

SN - 1046-2023

IS - 2

ER -

ID: 120790659