Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells. / Rasmussen, Mikkel Aabech; Hall, Vanessa Jane; Hyttel, Poul.

Epiblast stem cells: methods and protocols. ed. / Ramiro Alberio. Springer Science+Business Media, 2013. p. 185-198 (Methods in Molecular Biology, Vol. 1074).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Rasmussen, MA, Hall, VJ & Hyttel, P 2013, Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells. in R Alberio (ed.), Epiblast stem cells: methods and protocols. Springer Science+Business Media, Methods in Molecular Biology, vol. 1074, pp. 185-198. https://doi.org/10.1007/978-1-62703-628-3_14

APA

Rasmussen, M. A., Hall, V. J., & Hyttel, P. (2013). Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells. In R. Alberio (Ed.), Epiblast stem cells: methods and protocols (pp. 185-198). Springer Science+Business Media. Methods in Molecular Biology Vol. 1074 https://doi.org/10.1007/978-1-62703-628-3_14

Vancouver

Rasmussen MA, Hall VJ, Hyttel P. Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells. In Alberio R, editor, Epiblast stem cells: methods and protocols. Springer Science+Business Media. 2013. p. 185-198. (Methods in Molecular Biology, Vol. 1074). https://doi.org/10.1007/978-1-62703-628-3_14

Author

Rasmussen, Mikkel Aabech ; Hall, Vanessa Jane ; Hyttel, Poul. / Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells. Epiblast stem cells: methods and protocols. editor / Ramiro Alberio. Springer Science+Business Media, 2013. pp. 185-198 (Methods in Molecular Biology, Vol. 1074).

Bibtex

@inbook{903b184745794a86af0486ea1231cc05,
title = "Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells",
abstract = "The isolation and culture of neural progenitor cells (NPCs) from pluripotent stem cells has facilitated in vitro mechanistic studies of diseases related to the nervous system, as well as discovery of new medicine. In addition, NPCs are envisioned to play a crucial role in future cell replacement therapy. The pig has become recognized as an important large animal model and establishment of in vitro-derived porcine NPCs would allow for preclinical safety testing by transplantation in a porcine biomedical model. In this chapter, a detailed method for isolation and in vitro culture of porcine NPCs from porcine embryos or induced pluripotent stem cells is presented. The neural induction is performed in coculture and the isolation of rosette structures is carried out manually to ensure a homogenous population of NPCs. Using this method, multipotent NPCs can be obtained in approximately 1 month. The cells have the potential of long-term culture and the ability to differentiate into neural and glial cells.",
author = "Rasmussen, {Mikkel Aabech} and Hall, {Vanessa Jane} and Poul Hyttel",
year = "2013",
doi = "10.1007/978-1-62703-628-3_14",
language = "English",
isbn = "978-1-62703-627-6",
series = "Methods in Molecular Biology",
publisher = "Springer Science+Business Media",
pages = "185--198",
editor = "Ramiro Alberio",
booktitle = "Epiblast stem cells",
address = "Singapore",

}

RIS

TY - CHAP

T1 - Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells

AU - Rasmussen, Mikkel Aabech

AU - Hall, Vanessa Jane

AU - Hyttel, Poul

PY - 2013

Y1 - 2013

N2 - The isolation and culture of neural progenitor cells (NPCs) from pluripotent stem cells has facilitated in vitro mechanistic studies of diseases related to the nervous system, as well as discovery of new medicine. In addition, NPCs are envisioned to play a crucial role in future cell replacement therapy. The pig has become recognized as an important large animal model and establishment of in vitro-derived porcine NPCs would allow for preclinical safety testing by transplantation in a porcine biomedical model. In this chapter, a detailed method for isolation and in vitro culture of porcine NPCs from porcine embryos or induced pluripotent stem cells is presented. The neural induction is performed in coculture and the isolation of rosette structures is carried out manually to ensure a homogenous population of NPCs. Using this method, multipotent NPCs can be obtained in approximately 1 month. The cells have the potential of long-term culture and the ability to differentiate into neural and glial cells.

AB - The isolation and culture of neural progenitor cells (NPCs) from pluripotent stem cells has facilitated in vitro mechanistic studies of diseases related to the nervous system, as well as discovery of new medicine. In addition, NPCs are envisioned to play a crucial role in future cell replacement therapy. The pig has become recognized as an important large animal model and establishment of in vitro-derived porcine NPCs would allow for preclinical safety testing by transplantation in a porcine biomedical model. In this chapter, a detailed method for isolation and in vitro culture of porcine NPCs from porcine embryos or induced pluripotent stem cells is presented. The neural induction is performed in coculture and the isolation of rosette structures is carried out manually to ensure a homogenous population of NPCs. Using this method, multipotent NPCs can be obtained in approximately 1 month. The cells have the potential of long-term culture and the ability to differentiate into neural and glial cells.

U2 - 10.1007/978-1-62703-628-3_14

DO - 10.1007/978-1-62703-628-3_14

M3 - Book chapter

C2 - 23975814

SN - 978-1-62703-627-6

T3 - Methods in Molecular Biology

SP - 185

EP - 198

BT - Epiblast stem cells

A2 - Alberio, Ramiro

PB - Springer Science+Business Media

ER -

ID: 101691093