In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells

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In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells. / Blom, Lars; Poulsen, Lars K.

In: Journal of Immunological Methods, 29.08.2013.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Blom, L & Poulsen, LK 2013, 'In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells', Journal of Immunological Methods. https://doi.org/10.1016/j.jim.2013.08.008

APA

Blom, L., & Poulsen, L. K. (2013). In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells. Journal of Immunological Methods. https://doi.org/10.1016/j.jim.2013.08.008

Vancouver

Blom L, Poulsen LK. In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells. Journal of Immunological Methods. 2013 Aug 29. https://doi.org/10.1016/j.jim.2013.08.008

Author

Blom, Lars ; Poulsen, Lars K. / In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells. In: Journal of Immunological Methods. 2013.

Bibtex

@article{2b950dd2ff844b1e836ab6f65a9f89ee,
title = "In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of na{\"i}ve and memory CD4+ T cells",
abstract = "Robust in vitro systems are important in elucidating mechanisms regarding the heterogenetic nature of in vivo immune responses and contribute with knowledge to design good in vivo experiments. In this study, we show that initial Th1 and in particular Th2 polarization was negatively influenced by even small percentages (99% na{\"i}ve CD4+ T cells resulted in better Th1 and Th2 polarization with significant reduced fractions of IL-4+ and IFN-γ+ CD4+ T cells, respectively. Moreover, the Th2 primed >99% na{\"i}ve CD4+ T cells showed significantly higher ratio of IL-4+:IFN-γ+ (>4 fold) and GATA-3:+T-bet+ (>3 fold) CD4+ T cells when compared with the standard purified >90-95% na{\"i}ve CD4+ T cells primed under the same culture conditions. This suggests immunomagnetic bead separation, a low cost and easy available technique, with few modifications to the manufacturer's protocol as an attractive alternative for laboratories not having a cell sorter. Taken together, we report that it is essential to use rigorously purified (>99%) na{\"i}ve CD4+ T cells for optimal initial in vitro Th1 and Th2 priming.",
author = "Lars Blom and Poulsen, {Lars K.}",
note = "{\textcopyright} 2013.",
year = "2013",
month = aug,
day = "29",
doi = "10.1016/j.jim.2013.08.008",
language = "English",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells

AU - Blom, Lars

AU - Poulsen, Lars K.

N1 - © 2013.

PY - 2013/8/29

Y1 - 2013/8/29

N2 - Robust in vitro systems are important in elucidating mechanisms regarding the heterogenetic nature of in vivo immune responses and contribute with knowledge to design good in vivo experiments. In this study, we show that initial Th1 and in particular Th2 polarization was negatively influenced by even small percentages (99% naïve CD4+ T cells resulted in better Th1 and Th2 polarization with significant reduced fractions of IL-4+ and IFN-γ+ CD4+ T cells, respectively. Moreover, the Th2 primed >99% naïve CD4+ T cells showed significantly higher ratio of IL-4+:IFN-γ+ (>4 fold) and GATA-3:+T-bet+ (>3 fold) CD4+ T cells when compared with the standard purified >90-95% naïve CD4+ T cells primed under the same culture conditions. This suggests immunomagnetic bead separation, a low cost and easy available technique, with few modifications to the manufacturer's protocol as an attractive alternative for laboratories not having a cell sorter. Taken together, we report that it is essential to use rigorously purified (>99%) naïve CD4+ T cells for optimal initial in vitro Th1 and Th2 priming.

AB - Robust in vitro systems are important in elucidating mechanisms regarding the heterogenetic nature of in vivo immune responses and contribute with knowledge to design good in vivo experiments. In this study, we show that initial Th1 and in particular Th2 polarization was negatively influenced by even small percentages (99% naïve CD4+ T cells resulted in better Th1 and Th2 polarization with significant reduced fractions of IL-4+ and IFN-γ+ CD4+ T cells, respectively. Moreover, the Th2 primed >99% naïve CD4+ T cells showed significantly higher ratio of IL-4+:IFN-γ+ (>4 fold) and GATA-3:+T-bet+ (>3 fold) CD4+ T cells when compared with the standard purified >90-95% naïve CD4+ T cells primed under the same culture conditions. This suggests immunomagnetic bead separation, a low cost and easy available technique, with few modifications to the manufacturer's protocol as an attractive alternative for laboratories not having a cell sorter. Taken together, we report that it is essential to use rigorously purified (>99%) naïve CD4+ T cells for optimal initial in vitro Th1 and Th2 priming.

U2 - 10.1016/j.jim.2013.08.008

DO - 10.1016/j.jim.2013.08.008

M3 - Journal article

C2 - 23994109

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

ER -

ID: 50281541