TY - JOUR

T1 - Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters

T2 - Results of human intervention trials

AU - Ferk, Franziska

AU - Mišík, Miroslav

AU - Nersesyan, Armen

AU - Pichler, Christoph

AU - Jäger, Walter

AU - Szekeres, Thomas

AU - Marculescu, Rodrig

AU - Poulsen, Henrik E

AU - Henriksen, Trine

AU - Bono, Roberto

AU - Romanazzi, Valeria

AU - Al-Serori, Halh

AU - Biendl, Martin

AU - Wagner, Karl-Heinz

AU - Kundi, Michael

AU - Knasmüller, Siegfried

N1 - © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2016/4

Y1 - 2016/4

N2 - SCOPE: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out if XN causes alterations of health-related parameters in humans.METHODS AND RESULTS: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability in single cell gel electrophoresis assays in lymphocytes and of several health-related biomarkers. A decrease of oxidatively damaged purines and protection toward reactive oxygen species induced DNA damage was found after the consumption of the beverage; also the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism were not altered after the intervention.CONCLUSION: Taken together, our data indicate that low doses of XN protect humans against oxidative DNA damage.

AB - SCOPE: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out if XN causes alterations of health-related parameters in humans.METHODS AND RESULTS: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability in single cell gel electrophoresis assays in lymphocytes and of several health-related biomarkers. A decrease of oxidatively damaged purines and protection toward reactive oxygen species induced DNA damage was found after the consumption of the beverage; also the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism were not altered after the intervention.CONCLUSION: Taken together, our data indicate that low doses of XN protect humans against oxidative DNA damage.

KW - Adult

KW - DNA

KW - DNA Damage

KW - Deoxyguanosine

KW - Female

KW - Flavonoids

KW - Humans

KW - Male

KW - Propiophenones

KW - Protective Agents

KW - Single-Cell Analysis

KW - Journal Article

KW - Randomized Controlled Trial

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/mnfr.201500355

DO - 10.1002/mnfr.201500355

M3 - Journal article

C2 - 26840505

VL - 60

SP - 773

EP - 786

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 4

ER -