Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria

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Standard

Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria. / Hansen, K; Bangsborg, Jette Marie; Fjordvang, H; Pedersen, N S; Hindersson, P.

In: Infection and Immunity, Vol. 56, No. 8, 1988, p. 2047-53.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, K, Bangsborg, JM, Fjordvang, H, Pedersen, NS & Hindersson, P 1988, 'Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria', Infection and Immunity, vol. 56, no. 8, pp. 2047-53.

APA

Hansen, K., Bangsborg, J. M., Fjordvang, H., Pedersen, N. S., & Hindersson, P. (1988). Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria. Infection and Immunity, 56(8), 2047-53.

Vancouver

Hansen K, Bangsborg JM, Fjordvang H, Pedersen NS, Hindersson P. Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria. Infection and Immunity. 1988;56(8):2047-53.

Author

Hansen, K ; Bangsborg, Jette Marie ; Fjordvang, H ; Pedersen, N S ; Hindersson, P. / Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria. In: Infection and Immunity. 1988 ; Vol. 56, No. 8. pp. 2047-53.

Bibtex

@article{9fd1e5a56b5848bc9b52e00e9329ae66,
title = "Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria",
abstract = "By crossed immunoelectrophoresis and Western blotting (immunoblotting), it was shown that Borrelia burgdorferi expresses the 60-kilodalton Common Antigen (CA) that is cross-reactive with an equivalent antigen in a wide range of remotely related bacteria. B. burgdorferi CA is strongly immunogenic. A B. burgdorferi genomic library was constructed by using a plasmid cloning system. Escherichia coli recombinants were screened for expression of immunodominant B. burgdorferi antigens. One of the recombinant clones expressed the 60-kilodalton CA of B. burgdorferi. The DNA region encoding B. burgdorferi CA was localized on a 2.3-kilobase fragment of the plasmid pKH1. CA may have pathogenetic implications in Lyme borreliosis, since the CA of mycobacteria recently has been shown to play a role in the etiology of experimental autoimmune arthritis. The extensive cross-reactivity of this antigen may account for the low diagnostic specificity of the currently used serological tests in Lyme borreliosis.",
keywords = "Antigens, Bacterial, Borrelia, Cloning, Molecular, Cross Reactions, DNA Restriction Enzymes, Genes, Bacterial, Immunoelectrophoresis, Two-Dimensional, Lyme Disease, Molecular Weight",
author = "K Hansen and Bangsborg, {Jette Marie} and H Fjordvang and Pedersen, {N S} and P Hindersson",
year = "1988",
language = "English",
volume = "56",
pages = "2047--53",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "8",

}

RIS

TY - JOUR

T1 - Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria

AU - Hansen, K

AU - Bangsborg, Jette Marie

AU - Fjordvang, H

AU - Pedersen, N S

AU - Hindersson, P

PY - 1988

Y1 - 1988

N2 - By crossed immunoelectrophoresis and Western blotting (immunoblotting), it was shown that Borrelia burgdorferi expresses the 60-kilodalton Common Antigen (CA) that is cross-reactive with an equivalent antigen in a wide range of remotely related bacteria. B. burgdorferi CA is strongly immunogenic. A B. burgdorferi genomic library was constructed by using a plasmid cloning system. Escherichia coli recombinants were screened for expression of immunodominant B. burgdorferi antigens. One of the recombinant clones expressed the 60-kilodalton CA of B. burgdorferi. The DNA region encoding B. burgdorferi CA was localized on a 2.3-kilobase fragment of the plasmid pKH1. CA may have pathogenetic implications in Lyme borreliosis, since the CA of mycobacteria recently has been shown to play a role in the etiology of experimental autoimmune arthritis. The extensive cross-reactivity of this antigen may account for the low diagnostic specificity of the currently used serological tests in Lyme borreliosis.

AB - By crossed immunoelectrophoresis and Western blotting (immunoblotting), it was shown that Borrelia burgdorferi expresses the 60-kilodalton Common Antigen (CA) that is cross-reactive with an equivalent antigen in a wide range of remotely related bacteria. B. burgdorferi CA is strongly immunogenic. A B. burgdorferi genomic library was constructed by using a plasmid cloning system. Escherichia coli recombinants were screened for expression of immunodominant B. burgdorferi antigens. One of the recombinant clones expressed the 60-kilodalton CA of B. burgdorferi. The DNA region encoding B. burgdorferi CA was localized on a 2.3-kilobase fragment of the plasmid pKH1. CA may have pathogenetic implications in Lyme borreliosis, since the CA of mycobacteria recently has been shown to play a role in the etiology of experimental autoimmune arthritis. The extensive cross-reactivity of this antigen may account for the low diagnostic specificity of the currently used serological tests in Lyme borreliosis.

KW - Antigens, Bacterial

KW - Borrelia

KW - Cloning, Molecular

KW - Cross Reactions

KW - DNA Restriction Enzymes

KW - Genes, Bacterial

KW - Immunoelectrophoresis, Two-Dimensional

KW - Lyme Disease

KW - Molecular Weight

M3 - Journal article

C2 - 2840400

VL - 56

SP - 2047

EP - 2053

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 8

ER -

ID: 40334474