TY - JOUR

T1 - Human tandem-repeat-type galectins bind bacterial non-βGal polysaccharides

AU - Knirel, Yu A.

AU - Gabius, H.-J.

AU - Blixt, Klas Ola

AU - Rapoport, E.M.

AU - Khasbiullina, N.R.

AU - Shilova, N.V.

AU - Bovin, N.V.

PY - 2014

Y1 - 2014

N2 - Galectins are multifunctional effectors, for example acting as regulators of cell growth via protein-glycan interactions. The observation of capacity to kill bacteria for two tandem-repeat-type galectins, which target histo-blood epitopes toward this end (Stowell et al. Nat. Med. 16:295-301, 2010), prompted us to establish an array with bacterial polysaccharides. We addressed the question whether sugar determinants other than β-galactosides may be docking sites, using human galectins-4, -8, and -9. Positive controls with histo-blood group ABH-epitopes and the E. coli 086 polysaccharide ascertained the suitability of the set-up. Significant signal generation, depending on type of galectin and polysacchride, was obtained. Presence of cognate β-galactoside-related epitopes within a polysaccharide chain or its branch will not automatically establish binding properties, and structural constellations lacking galactosides, like rhamnan, were found to be active. These data establish the array as valuable screening tool, giving direction to further functional and structural studies.

AB - Galectins are multifunctional effectors, for example acting as regulators of cell growth via protein-glycan interactions. The observation of capacity to kill bacteria for two tandem-repeat-type galectins, which target histo-blood epitopes toward this end (Stowell et al. Nat. Med. 16:295-301, 2010), prompted us to establish an array with bacterial polysaccharides. We addressed the question whether sugar determinants other than β-galactosides may be docking sites, using human galectins-4, -8, and -9. Positive controls with histo-blood group ABH-epitopes and the E. coli 086 polysaccharide ascertained the suitability of the set-up. Significant signal generation, depending on type of galectin and polysacchride, was obtained. Presence of cognate β-galactoside-related epitopes within a polysaccharide chain or its branch will not automatically establish binding properties, and structural constellations lacking galactosides, like rhamnan, were found to be active. These data establish the array as valuable screening tool, giving direction to further functional and structural studies.

KW - Binding Sites

KW - Epitopes

KW - Galactosides

KW - Galectins

KW - Humans

KW - Polysaccharides, Bacterial

KW - Protein Binding

KW - Repetitive Sequences, Amino Acid

KW - Rhamnose

U2 - 10.1007/s10719-013-9497-3

DO - 10.1007/s10719-013-9497-3

M3 - Journal article

C2 - 24065176

VL - 31

SP - 7

EP - 12

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

SN - 0282-0080

IS - 1

ER -