Human anti-rhesus D IgG1 antibody produced in transgenic plants.
Research output: Contribution to journal › Journal article › Research › peer-review
Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.
Original language | English |
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Journal | Transgenic Research |
Volume | 11 |
Issue number | 2 |
Pages (from-to) | 115-22 |
Number of pages | 7 |
ISSN | 0962-8819 |
DOIs | |
Publication status | Published - 2002 |
Bibliographical note
Keywords: Agglutination Tests; Arabidopsis; Blotting, Western; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; Humans; Immunoglobulin G; Monocytes; Plants, Genetically Modified; Recombinant Proteins; Respiratory Burst; Rh-Hr Blood-Group System
ID: 6474713