Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria. / Hempel, Casper; Hyttel, Poul; Kurtzhals, Jørgen Al.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 34, No. 7, 07.2014, p. 1107-10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hempel, C, Hyttel, P & Kurtzhals, JA 2014, 'Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria', Journal of Cerebral Blood Flow and Metabolism, vol. 34, no. 7, pp. 1107-10. https://doi.org/10.1038/jcbfm.2014.79

APA

Hempel, C., Hyttel, P., & Kurtzhals, J. A. (2014). Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria. Journal of Cerebral Blood Flow and Metabolism, 34(7), 1107-10. https://doi.org/10.1038/jcbfm.2014.79

Vancouver

Hempel C, Hyttel P, Kurtzhals JA. Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria. Journal of Cerebral Blood Flow and Metabolism. 2014 Jul;34(7):1107-10. https://doi.org/10.1038/jcbfm.2014.79

Author

Hempel, Casper ; Hyttel, Poul ; Kurtzhals, Jørgen Al. / Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria. In: Journal of Cerebral Blood Flow and Metabolism. 2014 ; Vol. 34, No. 7. pp. 1107-10.

Bibtex

@article{a7642245d9b54d41b30cdc0d6fd70276,
title = "Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria",
abstract = "We hypothesized that the glycocalyx, which is important for endothelial integrity, is lost in severe malaria. C57BL/6 mice were infected with Plasmodium berghei ANKA, resulting in cerebral malaria, or P. chabaudi AS, resulting in uncomplicated malaria. We visualized the glycocalyx with transmission electron microscopy and measured circulating glycosaminoglycans by dot blot and ELISA. The glycocalyx was degraded in brain vasculature in cerebral and to a lesser degree uncomplicated malaria. It was affected on both intact and apoptotic endothelial cells. Circulating glycosaminoglycan levels suggested that glycocalyx disruption preceded cerebral manifestations. The contribution of this loss to pathogenesis should be studied further.",
author = "Casper Hempel and Poul Hyttel and Kurtzhals, {J{\o}rgen Al}",
year = "2014",
month = jul,
doi = "10.1038/jcbfm.2014.79",
language = "English",
volume = "34",
pages = "1107--10",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "SAGE Publications",
number = "7",

}

RIS

TY - JOUR

T1 - Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria

AU - Hempel, Casper

AU - Hyttel, Poul

AU - Kurtzhals, Jørgen Al

PY - 2014/7

Y1 - 2014/7

N2 - We hypothesized that the glycocalyx, which is important for endothelial integrity, is lost in severe malaria. C57BL/6 mice were infected with Plasmodium berghei ANKA, resulting in cerebral malaria, or P. chabaudi AS, resulting in uncomplicated malaria. We visualized the glycocalyx with transmission electron microscopy and measured circulating glycosaminoglycans by dot blot and ELISA. The glycocalyx was degraded in brain vasculature in cerebral and to a lesser degree uncomplicated malaria. It was affected on both intact and apoptotic endothelial cells. Circulating glycosaminoglycan levels suggested that glycocalyx disruption preceded cerebral manifestations. The contribution of this loss to pathogenesis should be studied further.

AB - We hypothesized that the glycocalyx, which is important for endothelial integrity, is lost in severe malaria. C57BL/6 mice were infected with Plasmodium berghei ANKA, resulting in cerebral malaria, or P. chabaudi AS, resulting in uncomplicated malaria. We visualized the glycocalyx with transmission electron microscopy and measured circulating glycosaminoglycans by dot blot and ELISA. The glycocalyx was degraded in brain vasculature in cerebral and to a lesser degree uncomplicated malaria. It was affected on both intact and apoptotic endothelial cells. Circulating glycosaminoglycan levels suggested that glycocalyx disruption preceded cerebral manifestations. The contribution of this loss to pathogenesis should be studied further.

U2 - 10.1038/jcbfm.2014.79

DO - 10.1038/jcbfm.2014.79

M3 - Journal article

C2 - 24756075

VL - 34

SP - 1107

EP - 1110

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 7

ER -

ID: 120389985