Drosha regulates gene expression independently of RNA cleavage function

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Drosha regulates gene expression independently of RNA cleavage function. / Gromak, Natalia; Dienstbier, Martin; Macias, Sara; Plass, Mireya; Eyras, Eduardo; Cáceres, Javier F.; Proudfoot, Nicholas J.

In: Cell Reports, Vol. 5, No. 6, 2013, p. 1499-510.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gromak, N, Dienstbier, M, Macias, S, Plass, M, Eyras, E, Cáceres, JF & Proudfoot, NJ 2013, 'Drosha regulates gene expression independently of RNA cleavage function', Cell Reports, vol. 5, no. 6, pp. 1499-510. https://doi.org/10.1016/j.celrep.2013.11.032

APA

Gromak, N., Dienstbier, M., Macias, S., Plass, M., Eyras, E., Cáceres, J. F., & Proudfoot, N. J. (2013). Drosha regulates gene expression independently of RNA cleavage function. Cell Reports, 5(6), 1499-510. https://doi.org/10.1016/j.celrep.2013.11.032

Vancouver

Gromak N, Dienstbier M, Macias S, Plass M, Eyras E, Cáceres JF et al. Drosha regulates gene expression independently of RNA cleavage function. Cell Reports. 2013;5(6):1499-510. https://doi.org/10.1016/j.celrep.2013.11.032

Author

Gromak, Natalia ; Dienstbier, Martin ; Macias, Sara ; Plass, Mireya ; Eyras, Eduardo ; Cáceres, Javier F. ; Proudfoot, Nicholas J. / Drosha regulates gene expression independently of RNA cleavage function. In: Cell Reports. 2013 ; Vol. 5, No. 6. pp. 1499-510.

Bibtex

@article{63cfb8ad7f044d768df2872ab4992dc2,
title = "Drosha regulates gene expression independently of RNA cleavage function",
abstract = "Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus. Using whole-genome ChIP-on-chip analysis, we demonstrate that, in addition to miRNA sequences, Drosha specifically binds promoter-proximal regions of many human genes in a transcription-dependent manner. This binding is not associated with miRNA production or RNA cleavage. Drosha knockdown in HeLa cells downregulated nascent gene transcription, resulting in a reduction of polyadenylated mRNA produced from these gene regions. Furthermore, we show that this function of Drosha is dependent on its N-terminal protein-interaction domain, which associates with the RNA-binding protein CBP80 and RNA Polymerase II. Consequently, we uncover a previously unsuspected RNA cleavage-independent function of Drosha in the regulation of human gene expression.",
author = "Natalia Gromak and Martin Dienstbier and Sara Macias and Mireya Plass and Eduardo Eyras and C{\'a}ceres, {Javier F.} and Proudfoot, {Nicholas J.}",
note = "Copyright {\textcopyright} 2013 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2013",
doi = "10.1016/j.celrep.2013.11.032",
language = "English",
volume = "5",
pages = "1499--510",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "6",

}

RIS

TY - JOUR

T1 - Drosha regulates gene expression independently of RNA cleavage function

AU - Gromak, Natalia

AU - Dienstbier, Martin

AU - Macias, Sara

AU - Plass, Mireya

AU - Eyras, Eduardo

AU - Cáceres, Javier F.

AU - Proudfoot, Nicholas J.

N1 - Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2013

Y1 - 2013

N2 - Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus. Using whole-genome ChIP-on-chip analysis, we demonstrate that, in addition to miRNA sequences, Drosha specifically binds promoter-proximal regions of many human genes in a transcription-dependent manner. This binding is not associated with miRNA production or RNA cleavage. Drosha knockdown in HeLa cells downregulated nascent gene transcription, resulting in a reduction of polyadenylated mRNA produced from these gene regions. Furthermore, we show that this function of Drosha is dependent on its N-terminal protein-interaction domain, which associates with the RNA-binding protein CBP80 and RNA Polymerase II. Consequently, we uncover a previously unsuspected RNA cleavage-independent function of Drosha in the regulation of human gene expression.

AB - Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus. Using whole-genome ChIP-on-chip analysis, we demonstrate that, in addition to miRNA sequences, Drosha specifically binds promoter-proximal regions of many human genes in a transcription-dependent manner. This binding is not associated with miRNA production or RNA cleavage. Drosha knockdown in HeLa cells downregulated nascent gene transcription, resulting in a reduction of polyadenylated mRNA produced from these gene regions. Furthermore, we show that this function of Drosha is dependent on its N-terminal protein-interaction domain, which associates with the RNA-binding protein CBP80 and RNA Polymerase II. Consequently, we uncover a previously unsuspected RNA cleavage-independent function of Drosha in the regulation of human gene expression.

U2 - 10.1016/j.celrep.2013.11.032

DO - 10.1016/j.celrep.2013.11.032

M3 - Journal article

C2 - 24360955

VL - 5

SP - 1499

EP - 1510

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 6

ER -

ID: 106792465