Discovery-based protein expression profiling identifies distinct subgroups and pathways in leiomyosarcomas
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Discovery-based protein expression profiling identifies distinct subgroups and pathways in leiomyosarcomas. / Kirik, Ufuk; Hansson, Karin; Krogh, Morten; Jönsson, Mats; Nilbert, Mef; James, Peter; Carneiro, Ana.
In: Molecular Cancer Research, Vol. 12, No. 12, 12.2014, p. 1729-1739.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Discovery-based protein expression profiling identifies distinct subgroups and pathways in leiomyosarcomas
AU - Kirik, Ufuk
AU - Hansson, Karin
AU - Krogh, Morten
AU - Jönsson, Mats
AU - Nilbert, Mef
AU - James, Peter
AU - Carneiro, Ana
N1 - ©2014 American Association for Cancer Research.
PY - 2014/12
Y1 - 2014/12
N2 - UNLABELLED: Soft tissue sarcomas (STS) are malignant tumors of mesenchymal origin. A substantial portion of these tumors exhibits complex karyotypes and lack characterized chromosomal aberrations. Owing to such properties, both histopathologic and molecular classification of these tumors has been a significant challenge. This study examines the protein expression of a large number of human STS, including subtype heterogeneity, using two-dimensional gel proteomics. In addition, detailed proteome profiles of a subset of pleomorphic STS specimens using an in-depth mass-spectrometry approach identified subgroups within the leiomyosarcomas with distinct protein expression patterns. Pathways analysis indicates that key biologic nodes like apoptosis, cytoskeleton remodeling, and telomere regulation are differentially regulated among these subgroups. Finally, investigating the similarities between protein expression of leiomyosarcomas and undifferentiated pleomorphic sarcomas (UPS) revealed similar protein expression profiles for these tumors, in comparison with pleomorphic leiomyosarcomas.IMPLICATIONS: These results suggest that UPS tumors share a similar lineage as leiomyosarcomas and are likely to originate from different stages of differentiation from mesenchymal stem cells to smooth muscle cells.
AB - UNLABELLED: Soft tissue sarcomas (STS) are malignant tumors of mesenchymal origin. A substantial portion of these tumors exhibits complex karyotypes and lack characterized chromosomal aberrations. Owing to such properties, both histopathologic and molecular classification of these tumors has been a significant challenge. This study examines the protein expression of a large number of human STS, including subtype heterogeneity, using two-dimensional gel proteomics. In addition, detailed proteome profiles of a subset of pleomorphic STS specimens using an in-depth mass-spectrometry approach identified subgroups within the leiomyosarcomas with distinct protein expression patterns. Pathways analysis indicates that key biologic nodes like apoptosis, cytoskeleton remodeling, and telomere regulation are differentially regulated among these subgroups. Finally, investigating the similarities between protein expression of leiomyosarcomas and undifferentiated pleomorphic sarcomas (UPS) revealed similar protein expression profiles for these tumors, in comparison with pleomorphic leiomyosarcomas.IMPLICATIONS: These results suggest that UPS tumors share a similar lineage as leiomyosarcomas and are likely to originate from different stages of differentiation from mesenchymal stem cells to smooth muscle cells.
U2 - 10.1158/1541-7786.MCR-14-0072
DO - 10.1158/1541-7786.MCR-14-0072
M3 - Journal article
C2 - 25069693
VL - 12
SP - 1729
EP - 1739
JO - Molecular Cancer Research
JF - Molecular Cancer Research
SN - 1541-7786
IS - 12
ER -
ID: 137744195