Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans

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Standard

Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids : a method applicable in humans. / Holm, Lars; O'Rourke, Bruce; Ebenstein, David; Toth, Michael J; Bechshoeft, Rasmus; Holstein-Rathlou, Niels-Henrik; Kjær, Michael; Matthews, Dwight E.

In: A J P: Endocrinology and Metabolism (Online), Vol. 304, No. 8, 2013, p. E895-E907.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Holm, L, O'Rourke, B, Ebenstein, D, Toth, MJ, Bechshoeft, R, Holstein-Rathlou, N-H, Kjær, M & Matthews, DE 2013, 'Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans', A J P: Endocrinology and Metabolism (Online), vol. 304, no. 8, pp. E895-E907. https://doi.org/10.1152/ajpendo.00579.2012

APA

Holm, L., O'Rourke, B., Ebenstein, D., Toth, M. J., Bechshoeft, R., Holstein-Rathlou, N-H., Kjær, M., & Matthews, D. E. (2013). Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans. A J P: Endocrinology and Metabolism (Online), 304(8), E895-E907. https://doi.org/10.1152/ajpendo.00579.2012

Vancouver

Holm L, O'Rourke B, Ebenstein D, Toth MJ, Bechshoeft R, Holstein-Rathlou N-H et al. Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans. A J P: Endocrinology and Metabolism (Online). 2013;304(8):E895-E907. https://doi.org/10.1152/ajpendo.00579.2012

Author

Holm, Lars ; O'Rourke, Bruce ; Ebenstein, David ; Toth, Michael J ; Bechshoeft, Rasmus ; Holstein-Rathlou, Niels-Henrik ; Kjær, Michael ; Matthews, Dwight E. / Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids : a method applicable in humans. In: A J P: Endocrinology and Metabolism (Online). 2013 ; Vol. 304, No. 8. pp. E895-E907.

Bibtex

@article{01ca8e25a26941ad9f99bf647d273063,
title = "Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans",
abstract = "A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest. ",
author = "Lars Holm and Bruce O'Rourke and David Ebenstein and Toth, {Michael J} and Rasmus Bechshoeft and Niels-Henrik Holstein-Rathlou and Michael Kj{\ae}r and Matthews, {Dwight E}",
year = "2013",
doi = "10.1152/ajpendo.00579.2012",
language = "English",
volume = "304",
pages = "E895--E907",
journal = "A J P: Endocrinology and Metabolism (Online)",
issn = "1522-1555",
publisher = "American Physiological Society",
number = "8",

}

RIS

TY - JOUR

T1 - Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids

T2 - a method applicable in humans

AU - Holm, Lars

AU - O'Rourke, Bruce

AU - Ebenstein, David

AU - Toth, Michael J

AU - Bechshoeft, Rasmus

AU - Holstein-Rathlou, Niels-Henrik

AU - Kjær, Michael

AU - Matthews, Dwight E

PY - 2013

Y1 - 2013

N2 - A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.

AB - A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.

U2 - 10.1152/ajpendo.00579.2012

DO - 10.1152/ajpendo.00579.2012

M3 - Journal article

VL - 304

SP - E895-E907

JO - A J P: Endocrinology and Metabolism (Online)

JF - A J P: Endocrinology and Metabolism (Online)

SN - 1522-1555

IS - 8

ER -

ID: 119412018