Contribution of Kv7 channels to natriuretic peptide mediated vasodilation in normal and hypertensive rats
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Contribution of Kv7 channels to natriuretic peptide mediated vasodilation in normal and hypertensive rats. / Stott, Jennifer B; Barrese, Vincenzo; Jepps, Thomas Andrew; Leighton, Emma V; Greenwood, Iain A.
In: Hypertension, Vol. 65, No. 3, 03.2015, p. 676-82.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Contribution of Kv7 channels to natriuretic peptide mediated vasodilation in normal and hypertensive rats
AU - Stott, Jennifer B
AU - Barrese, Vincenzo
AU - Jepps, Thomas Andrew
AU - Leighton, Emma V
AU - Greenwood, Iain A
N1 - © 2014 American Heart Association, Inc.
PY - 2015/3
Y1 - 2015/3
N2 - The Kv7 family of voltage-gated potassium channels are expressed within the vasculature where they are key regulators of vascular tone and mediate cAMP-linked endogenous vasodilator responses, a pathway that is compromised in hypertension. However, the role of Kv7 channels in non-cAMP-linked vasodilator pathways has not been investigated. Natriuretic peptides are potent vasodilators, which operate primarily through the activation of a cGMP-dependent signaling pathway. This study investigated the putative role of Kv7 channels in natriuretic peptide-dependent relaxations in the vasculature of normal and hypertensive animals. Relaxant responses of rat aorta to both atrial and C-type natriuretic peptides and the nitric oxide donor sodium nitroprusside were impaired by the Kv7 blocker linopirdine (10 μmol/L) but not by the Kv7.1-specific blocker HMR1556 (10 μmol/L) and other K(+) channel blockers. In contrast, only the atrial natriuretic peptide response was sensitive to linopirdine in the renal artery. These Kv7-mediated responses were attenuated in arteries from hypertensive rats. Quantitative polymerase chain reaction showed that A- and B-type natriuretic peptide receptors were expressed at high levels in the aorta and renal artery from normal and spontaneously hypertensive rats. This study provides the first evidence that natriuretic peptide responses are impaired in hypertension and that recruitment of Kv7 channels is a key component of natriuretic peptide-dependent vasodilations.
AB - The Kv7 family of voltage-gated potassium channels are expressed within the vasculature where they are key regulators of vascular tone and mediate cAMP-linked endogenous vasodilator responses, a pathway that is compromised in hypertension. However, the role of Kv7 channels in non-cAMP-linked vasodilator pathways has not been investigated. Natriuretic peptides are potent vasodilators, which operate primarily through the activation of a cGMP-dependent signaling pathway. This study investigated the putative role of Kv7 channels in natriuretic peptide-dependent relaxations in the vasculature of normal and hypertensive animals. Relaxant responses of rat aorta to both atrial and C-type natriuretic peptides and the nitric oxide donor sodium nitroprusside were impaired by the Kv7 blocker linopirdine (10 μmol/L) but not by the Kv7.1-specific blocker HMR1556 (10 μmol/L) and other K(+) channel blockers. In contrast, only the atrial natriuretic peptide response was sensitive to linopirdine in the renal artery. These Kv7-mediated responses were attenuated in arteries from hypertensive rats. Quantitative polymerase chain reaction showed that A- and B-type natriuretic peptide receptors were expressed at high levels in the aorta and renal artery from normal and spontaneously hypertensive rats. This study provides the first evidence that natriuretic peptide responses are impaired in hypertension and that recruitment of Kv7 channels is a key component of natriuretic peptide-dependent vasodilations.
KW - Animals
KW - Aorta
KW - Cyclic GMP
KW - Disease Models, Animal
KW - Hypertension
KW - KCNQ Potassium Channels
KW - Muscle, Smooth, Vascular
KW - Natriuretic Peptides
KW - Nitroprusside
KW - Potassium Channel Blockers
KW - Rats
KW - Rats, Inbred SHR
KW - Rats, Wistar
KW - Renal Artery
KW - Signal Transduction
KW - Vasodilation
U2 - 10.1161/HYPERTENSIONAHA.114.04373
DO - 10.1161/HYPERTENSIONAHA.114.04373
M3 - Journal article
C2 - 25547342
VL - 65
SP - 676
EP - 682
JO - Hypertension
JF - Hypertension
SN - 0194-911X
IS - 3
ER -
ID: 137319567