Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries
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Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries. / Haanes, Kristian Agmund; Edvinsson, Lars.
In: FEBS Letters, Vol. 588, No. 17, 25.08.2014, p. 2936-2943.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries
AU - Haanes, Kristian Agmund
AU - Edvinsson, Lars
N1 - Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
PY - 2014/8/25
Y1 - 2014/8/25
N2 - Extracellular UDP-glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP-glucose causes contraction in mouse coronary and basilar arteries. The EC50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP-glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.
AB - Extracellular UDP-glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP-glucose causes contraction in mouse coronary and basilar arteries. The EC50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP-glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.
KW - Animals
KW - Cerebral Arteries
KW - Coronary Vessels
KW - Female
KW - In Vitro Techniques
KW - Male
KW - Mice
KW - Mice, Inbred BALB C
KW - Muscle Contraction
KW - Pertussis Toxin
KW - Receptors, Purinergic P2Y
KW - Up-Regulation
KW - Uridine Diphosphate Glucose
U2 - 10.1016/j.febslet.2014.05.044
DO - 10.1016/j.febslet.2014.05.044
M3 - Journal article
C2 - 24911208
VL - 588
SP - 2936
EP - 2943
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 17
ER -
ID: 138221389