Approaches to link RNA secondary structures with splicing regulation
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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Approaches to link RNA secondary structures with splicing regulation. / Plass, Mireya; Eyras, Eduardo.
Spliceosomal Pre-mRNA Splicing. ed. / Klemens J. Hertel . Vol. 1126 Springer Science+Business Media, 2014. p. 341-356 (Methods in Molecular Biology, Vol. 1126).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Approaches to link RNA secondary structures with splicing regulation
AU - Plass, Mireya
AU - Eyras, Eduardo
PY - 2014
Y1 - 2014
N2 - In higher eukaryotes, alternative splicing is usually regulated by protein factors, which bind to the pre-mRNA and affect the recognition of splicing signals. There is recent evidence that the secondary structure of the pre-mRNA may also play an important role in this process, either by facilitating or hindering the interaction with factors and small nuclear ribonucleoproteins (snRNPs) that regulate splicing. Moreover, the secondary structure could play a fundamental role in the splicing of yeast species, which lack many of the regulatory splicing factors present in metazoans. This chapter describes the steps in the analysis of the secondary structure of the pre-mRNA and its possible relation to splicing. As a working example, we use the case of yeast and the problem of the recognition of the 3' splice site (3'ss).
AB - In higher eukaryotes, alternative splicing is usually regulated by protein factors, which bind to the pre-mRNA and affect the recognition of splicing signals. There is recent evidence that the secondary structure of the pre-mRNA may also play an important role in this process, either by facilitating or hindering the interaction with factors and small nuclear ribonucleoproteins (snRNPs) that regulate splicing. Moreover, the secondary structure could play a fundamental role in the splicing of yeast species, which lack many of the regulatory splicing factors present in metazoans. This chapter describes the steps in the analysis of the secondary structure of the pre-mRNA and its possible relation to splicing. As a working example, we use the case of yeast and the problem of the recognition of the 3' splice site (3'ss).
U2 - 10.1007/978-1-62703-980-2_25
DO - 10.1007/978-1-62703-980-2_25
M3 - Book chapter
C2 - 24549676
SN - 978-1-62703-979-6
VL - 1126
T3 - Methods in Molecular Biology
SP - 341
EP - 356
BT - Spliceosomal Pre-mRNA Splicing
A2 - Hertel , Klemens J.
PB - Springer Science+Business Media
ER -
ID: 120338791