Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation
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Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation. / Simonsen, Stine; Thyssen, Jacob P.; Heegaard, Steffen; Kezic, Sanja; Skov, Lone.
In: Acta Dermato-Venereologica, Vol. 97, No. 7, 2017, p. 797-801.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Expression of filaggrin and its degradation products in human skin following erythemal doses of ultraviolet B irradiation
AU - Simonsen, Stine
AU - Thyssen, Jacob P.
AU - Heegaard, Steffen
AU - Kezic, Sanja
AU - Skov, Lone
PY - 2017
Y1 - 2017
N2 - Epidermal filaggrin level is affected by ultraviolet B irradiation in animal and experimental models. This study examined the effect of ultraviolet B irradiation on epidermal filaggrin and natural moisturizing factors in vivo in healthy adults (n = 22). Participants were irradiated with 2 minimal erythema doses of ultraviolet B on the skin. Biopsies and tape strips were collected from skin irradiated 24 and 72 h earlier and from nonirradiated skin (control). Real-time quantitative PCR on skin biopsies showed significantly reduced profilaggrin mRNA expression 24 h after irradiation (mean relative mRNA expression ± standard deviation: control, 3.86 ± 2.06 vs. 24 h, 1.52 ± 0.640; p = 0.02; n = 8). Immunohistochemistry showed aberrant spatial distribution of filaggrin protein 72 h after irradiation (n = 3). High-pressure liquid chromatography of tape extracts showed no change in mean total natural moisturizing factor levels after irradiation, but mean trans-urocanic acid was significantly reduced, as expected (n = 8). In conclusion, erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo.
AB - Epidermal filaggrin level is affected by ultraviolet B irradiation in animal and experimental models. This study examined the effect of ultraviolet B irradiation on epidermal filaggrin and natural moisturizing factors in vivo in healthy adults (n = 22). Participants were irradiated with 2 minimal erythema doses of ultraviolet B on the skin. Biopsies and tape strips were collected from skin irradiated 24 and 72 h earlier and from nonirradiated skin (control). Real-time quantitative PCR on skin biopsies showed significantly reduced profilaggrin mRNA expression 24 h after irradiation (mean relative mRNA expression ± standard deviation: control, 3.86 ± 2.06 vs. 24 h, 1.52 ± 0.640; p = 0.02; n = 8). Immunohistochemistry showed aberrant spatial distribution of filaggrin protein 72 h after irradiation (n = 3). High-pressure liquid chromatography of tape extracts showed no change in mean total natural moisturizing factor levels after irradiation, but mean trans-urocanic acid was significantly reduced, as expected (n = 8). In conclusion, erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo.
KW - Acute inflammation
KW - Filaggrin
KW - Human
KW - Ultraviolet B
U2 - 10.2340/00015555-2662
DO - 10.2340/00015555-2662
M3 - Journal article
C2 - 28358172
AN - SCOPUS:85021887138
VL - 97
SP - 797
EP - 801
JO - Acta Dermato-Venereologica
JF - Acta Dermato-Venereologica
SN - 0001-5555
IS - 7
ER -
ID: 182093142