An Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples
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An Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples. / Bag, Satyabrata; Saha, Bipasa; Mehta, Ojasvi; Anbumani, D; Kumar, Naveen; Dayal, Mayanka; Pant, Archana; Kumar, Pawan; Saxena, Shruti; Allin, Kristine H; Hansen, Torben; Arumugam, Manimozhiyan; Vestergaard, Henrik; Pedersen, Oluf; Pereira, Verima; Abraham, Philip; Tripathi, Reva; Wadhwa, Nitya; Bhatnagar, Shinjini; Prakash, Visvanathan Gnana; Radha, Venkatesan; Anjana, R M; Mohan, V; Takeda, Kiyoshi; Kurakawa, Takashi; Nair, G Balakrish; Das, Bhabatosh.
In: Scientific Reports, Vol. 6, 26775, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - An Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples
AU - Bag, Satyabrata
AU - Saha, Bipasa
AU - Mehta, Ojasvi
AU - Anbumani, D
AU - Kumar, Naveen
AU - Dayal, Mayanka
AU - Pant, Archana
AU - Kumar, Pawan
AU - Saxena, Shruti
AU - Allin, Kristine H
AU - Hansen, Torben
AU - Arumugam, Manimozhiyan
AU - Vestergaard, Henrik
AU - Pedersen, Oluf
AU - Pereira, Verima
AU - Abraham, Philip
AU - Tripathi, Reva
AU - Wadhwa, Nitya
AU - Bhatnagar, Shinjini
AU - Prakash, Visvanathan Gnana
AU - Radha, Venkatesan
AU - Anjana, R M
AU - Mohan, V
AU - Takeda, Kiyoshi
AU - Kurakawa, Takashi
AU - Nair, G Balakrish
AU - Das, Bhabatosh
PY - 2016
Y1 - 2016
N2 - To explore the natural microbial community of any ecosystems by high-resolution molecular approaches including next generation sequencing, it is extremely important to develop a sensitive and reproducible DNA extraction method that facilitate isolation of microbial DNA of sufficient purity and quantity from culturable and uncultured microbial species living in that environment. Proper lysis of heterogeneous community microbial cells without damaging their genomes is a major challenge. In this study, we have developed an improved method for extraction of community DNA from different environmental and human origin samples. We introduced a combination of physical, chemical and mechanical lysis methods for proper lysis of microbial inhabitants. The community microbial DNA was precipitated by using salt and organic solvent. Both the quality and quantity of isolated DNA was compared with the existing methodologies and the supremacy of our method was confirmed. Maximum recovery of genomic DNA in the absence of substantial amount of impurities made the method convenient for nucleic acid extraction. The nucleic acids obtained using this method are suitable for different downstream applications. This improved method has been named as the THSTI method to depict the Institute where the method was developed.
AB - To explore the natural microbial community of any ecosystems by high-resolution molecular approaches including next generation sequencing, it is extremely important to develop a sensitive and reproducible DNA extraction method that facilitate isolation of microbial DNA of sufficient purity and quantity from culturable and uncultured microbial species living in that environment. Proper lysis of heterogeneous community microbial cells without damaging their genomes is a major challenge. In this study, we have developed an improved method for extraction of community DNA from different environmental and human origin samples. We introduced a combination of physical, chemical and mechanical lysis methods for proper lysis of microbial inhabitants. The community microbial DNA was precipitated by using salt and organic solvent. Both the quality and quantity of isolated DNA was compared with the existing methodologies and the supremacy of our method was confirmed. Maximum recovery of genomic DNA in the absence of substantial amount of impurities made the method convenient for nucleic acid extraction. The nucleic acids obtained using this method are suitable for different downstream applications. This improved method has been named as the THSTI method to depict the Institute where the method was developed.
U2 - 10.1038/srep26775
DO - 10.1038/srep26775
M3 - Journal article
C2 - 27240745
VL - 6
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 26775
ER -
ID: 162125159