A VAR2CSA:CSP conjugate capable of inducing dual specificity antibody responses
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- A VAR2CSA:CSP conjugate capable of inducing dual specificity antibody responses
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Background: Vaccine antigens targeting specific P. falciparum parasite stages are under pre-clinical and clinical development. It seems plausible that vaccine with multiple specificities will offer higher protection. With this hypothesis, we exploited the Spy-Tag/SpyCatcher conjugation system to make a, post expression, dual antigen conjugate vaccine, comprising two clinically tested antigen candidates (CSP and VAR2CSA).
Methods: The DBL1x-DBL2x-ID2a region of VAR2CSA was genetically fused with SpyTag at N-terminus. The full-length CSP antigen was genetically fused to C-terminal SpyCatcher peptide. The covalent interaction between SpyTag/SpyCatcher enables the formation of DBL1x-DBL2x-ID2a:CSP conjugate vaccine. Immunogenicity and quality of antibody responses induced by the conjugate vaccine, as well as a control CSP-SpyCatcher vaccine, was tested in BALB/c mice.
Results: Serum samples obtained from mice immunized with the conjugate vaccine were able to recognize both untagged DBL1x-DBL2x-ID2a as well as CSP antigen. Moreover, the geometric mean anti-CSP antibody titer was 1.9-fold higher in serum (at day 35 and 55 post-first immunization) from mice immunized with the conjugate vaccine, as compared to mice receiving the control vaccine.
Conclusion: The data obtained in this study serves as proof-of-concept for the simultaneous induction of antibodies directed against individual antigen components in a dual stage anti-malaria vaccine.
Original language | English |
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Journal | African Health Sciences |
Volume | 17 |
Issue number | 2 |
Pages (from-to) | 373-381 |
Number of pages | 9 |
ISSN | 1680-6905 |
DOIs | |
Publication status | Published - 2017 |
- Bacterial superglue, Circumsporozoite protein, CSP-SpyCatcher, DBL1x-DBL2x-ID2a:CSP conjugate, Malaria vaccine, SpyTag-DBL1x-DBL2x-ID2a, VAR2CSA
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