Stability of glucagon-like peptide-1 and glucagon in human plasma

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Stability of glucagon-like peptide-1 and glucagon in human plasma. / Albrechtsen, Nicolai Jacob Wewer; Bak, Monika Judyta; Hartmann, Bolette; Christensen, Louise Wulff; Kuhre, Rune E; Deacon, Carolyn; Holst, Jens Juul.

I: Endocrine Connections, Bind 4, Nr. 1, 16.01.2015, s. 50-57.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Albrechtsen, NJW, Bak, MJ, Hartmann, B, Christensen, LW, Kuhre, RE, Deacon, C & Holst, JJ 2015, 'Stability of glucagon-like peptide-1 and glucagon in human plasma', Endocrine Connections, bind 4, nr. 1, s. 50-57. https://doi.org/10.1530/EC-14-0126

APA

Albrechtsen, N. J. W., Bak, M. J., Hartmann, B., Christensen, L. W., Kuhre, R. E., Deacon, C., & Holst, J. J. (2015). Stability of glucagon-like peptide-1 and glucagon in human plasma. Endocrine Connections, 4(1), 50-57. https://doi.org/10.1530/EC-14-0126

Vancouver

Albrechtsen NJW, Bak MJ, Hartmann B, Christensen LW, Kuhre RE, Deacon C o.a. Stability of glucagon-like peptide-1 and glucagon in human plasma. Endocrine Connections. 2015 jan 16;4(1):50-57. https://doi.org/10.1530/EC-14-0126

Author

Albrechtsen, Nicolai Jacob Wewer ; Bak, Monika Judyta ; Hartmann, Bolette ; Christensen, Louise Wulff ; Kuhre, Rune E ; Deacon, Carolyn ; Holst, Jens Juul. / Stability of glucagon-like peptide-1 and glucagon in human plasma. I: Endocrine Connections. 2015 ; Bind 4, Nr. 1. s. 50-57.

Bibtex

@article{15017843c7aa4702b9e793118b438fd7,
title = "Stability of glucagon-like peptide-1 and glucagon in human plasma",
abstract = "To investigate the stability of glucagon-like peptide-1(GLP-1) and glucagon in plasma under short- and long-term storage conditions. Methods: Pooled human plasma (n=20), to which a dipeptidyl peptidase 4 (DPP-4) inhibitor and aprotinin were added, was spiked with synthetic GLP-1 (intact, 7-36NH2 as well as the primary metabolite, GLP-1 9-36 NH2) or glucagon. Peptide recoveries were measured in samples kept for 1 and 3 hours at room temperature or on ice, treated with various enzyme inhibitors, after up to 3 thawing/refreezing cycles, and after storage at -20 and -80C for up to 1 year. Results: Recoveries were unaffected by freezing cycles or if plasma was stored on ice for up to 3 hours, but were impaired when samples stood at RT for more than 1 hour. Recovery of intact GLP-1 increased by addition of a DPP-4 inhibitor (no ice), but was not further improved by neutral endopeptidase 24.11 inhibitor or an inhibitor cocktail. GLP-1, but not glucagon, was stable for at least 1 year. Surprisingly, the recovery of glucagon was reduced by almost 50{\%} by freezing compared to immediate analysis, regardless of storage time. Conclusion: Plasma handling procedures can significantly influence results of subsequent hormone analysis. Our data support addition of DPP-4 inhibitor for GLP-1 measurement as well as cooling on ice of both GLP-1 and glucagon. Freeze/thaw cycles did not significantly affect stability of GLP-1 or glucagon. Long term storage may affect glucagon levels regardless of storage temperature and results should be interpreted with caution.",
author = "Albrechtsen, {Nicolai Jacob Wewer} and Bak, {Monika Judyta} and Bolette Hartmann and Christensen, {Louise Wulff} and Kuhre, {Rune E} and Carolyn Deacon and Holst, {Jens Juul}",
year = "2015",
month = "1",
day = "16",
doi = "10.1530/EC-14-0126",
language = "English",
volume = "4",
pages = "50--57",
journal = "Endocrine Connections",
issn = "2049-3614",
publisher = "BioScientifica Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Stability of glucagon-like peptide-1 and glucagon in human plasma

AU - Albrechtsen, Nicolai Jacob Wewer

AU - Bak, Monika Judyta

AU - Hartmann, Bolette

AU - Christensen, Louise Wulff

AU - Kuhre, Rune E

AU - Deacon, Carolyn

AU - Holst, Jens Juul

PY - 2015/1/16

Y1 - 2015/1/16

N2 - To investigate the stability of glucagon-like peptide-1(GLP-1) and glucagon in plasma under short- and long-term storage conditions. Methods: Pooled human plasma (n=20), to which a dipeptidyl peptidase 4 (DPP-4) inhibitor and aprotinin were added, was spiked with synthetic GLP-1 (intact, 7-36NH2 as well as the primary metabolite, GLP-1 9-36 NH2) or glucagon. Peptide recoveries were measured in samples kept for 1 and 3 hours at room temperature or on ice, treated with various enzyme inhibitors, after up to 3 thawing/refreezing cycles, and after storage at -20 and -80C for up to 1 year. Results: Recoveries were unaffected by freezing cycles or if plasma was stored on ice for up to 3 hours, but were impaired when samples stood at RT for more than 1 hour. Recovery of intact GLP-1 increased by addition of a DPP-4 inhibitor (no ice), but was not further improved by neutral endopeptidase 24.11 inhibitor or an inhibitor cocktail. GLP-1, but not glucagon, was stable for at least 1 year. Surprisingly, the recovery of glucagon was reduced by almost 50% by freezing compared to immediate analysis, regardless of storage time. Conclusion: Plasma handling procedures can significantly influence results of subsequent hormone analysis. Our data support addition of DPP-4 inhibitor for GLP-1 measurement as well as cooling on ice of both GLP-1 and glucagon. Freeze/thaw cycles did not significantly affect stability of GLP-1 or glucagon. Long term storage may affect glucagon levels regardless of storage temperature and results should be interpreted with caution.

AB - To investigate the stability of glucagon-like peptide-1(GLP-1) and glucagon in plasma under short- and long-term storage conditions. Methods: Pooled human plasma (n=20), to which a dipeptidyl peptidase 4 (DPP-4) inhibitor and aprotinin were added, was spiked with synthetic GLP-1 (intact, 7-36NH2 as well as the primary metabolite, GLP-1 9-36 NH2) or glucagon. Peptide recoveries were measured in samples kept for 1 and 3 hours at room temperature or on ice, treated with various enzyme inhibitors, after up to 3 thawing/refreezing cycles, and after storage at -20 and -80C for up to 1 year. Results: Recoveries were unaffected by freezing cycles or if plasma was stored on ice for up to 3 hours, but were impaired when samples stood at RT for more than 1 hour. Recovery of intact GLP-1 increased by addition of a DPP-4 inhibitor (no ice), but was not further improved by neutral endopeptidase 24.11 inhibitor or an inhibitor cocktail. GLP-1, but not glucagon, was stable for at least 1 year. Surprisingly, the recovery of glucagon was reduced by almost 50% by freezing compared to immediate analysis, regardless of storage time. Conclusion: Plasma handling procedures can significantly influence results of subsequent hormone analysis. Our data support addition of DPP-4 inhibitor for GLP-1 measurement as well as cooling on ice of both GLP-1 and glucagon. Freeze/thaw cycles did not significantly affect stability of GLP-1 or glucagon. Long term storage may affect glucagon levels regardless of storage temperature and results should be interpreted with caution.

U2 - 10.1530/EC-14-0126

DO - 10.1530/EC-14-0126

M3 - Journal article

C2 - 25596009

VL - 4

SP - 50

EP - 57

JO - Endocrine Connections

JF - Endocrine Connections

SN - 2049-3614

IS - 1

ER -

ID: 130200595