Retinal shows its true colours: Photoisomerization action spectra of mobility-selected isomers of the retinal protonated Schiff base
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Retinal shows its true colours : Photoisomerization action spectra of mobility-selected isomers of the retinal protonated Schiff base. / Coughlan, N. J.A.; Adamson, B. D.; Gamon, L.; Catani, K.; Bieske, E. J.
I: Physical Chemistry Chemical Physics, Bind 17, Nr. 35, 05.08.2015, s. 22623-22631.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Retinal shows its true colours
T2 - Photoisomerization action spectra of mobility-selected isomers of the retinal protonated Schiff base
AU - Coughlan, N. J.A.
AU - Adamson, B. D.
AU - Gamon, L.
AU - Catani, K.
AU - Bieske, E. J.
PY - 2015/8/5
Y1 - 2015/8/5
N2 - Retinal is one of Nature's most important and widespread chromophores, exhibiting remarkable versatility in its function and spectral response, depending on its protein environment. Reliable spectroscopic and photochemical data for the isolated retinal molecule are essential for calibrating theoretical approaches that seek to model retinal's behaviour in complex protein environments. However, due to low densities and possible co-existence of multiple isomers, retinal is a challenging target for gas-phase investigations. Here, the photoisomerization behaviour of the trans isomer of the retinal protonated Schiff base (RPSB) is investigated in the gas phase by irradiating mobility-selected RPSB ions with tunable light in a tandem ion mobility spectrometer. trans RPSB ions are converted to single cis isomers and also more compact isomers through irradiation with visible light. The S1 ← S0 photoisomerization action spectrum of trans RPSB, obtained by monitoring production of cis isomers as a function of wavelength, exhibits a single well-defined peak with a maximum at 618 ± 5 nm. Corresponding action spectra of cis RPSB isomers exhibit broader peaks, conclusively demonstrating an isomeric dependence for the RPSB spectrum in the gas phase.
AB - Retinal is one of Nature's most important and widespread chromophores, exhibiting remarkable versatility in its function and spectral response, depending on its protein environment. Reliable spectroscopic and photochemical data for the isolated retinal molecule are essential for calibrating theoretical approaches that seek to model retinal's behaviour in complex protein environments. However, due to low densities and possible co-existence of multiple isomers, retinal is a challenging target for gas-phase investigations. Here, the photoisomerization behaviour of the trans isomer of the retinal protonated Schiff base (RPSB) is investigated in the gas phase by irradiating mobility-selected RPSB ions with tunable light in a tandem ion mobility spectrometer. trans RPSB ions are converted to single cis isomers and also more compact isomers through irradiation with visible light. The S1 ← S0 photoisomerization action spectrum of trans RPSB, obtained by monitoring production of cis isomers as a function of wavelength, exhibits a single well-defined peak with a maximum at 618 ± 5 nm. Corresponding action spectra of cis RPSB isomers exhibit broader peaks, conclusively demonstrating an isomeric dependence for the RPSB spectrum in the gas phase.
UR - http://www.scopus.com/inward/record.url?scp=84940434565&partnerID=8YFLogxK
U2 - 10.1039/c5cp03611a
DO - 10.1039/c5cp03611a
M3 - Journal article
C2 - 26280514
AN - SCOPUS:84940434565
VL - 17
SP - 22623
EP - 22631
JO - Physical Chemistry Chemical Physics
JF - Physical Chemistry Chemical Physics
SN - 1463-9076
IS - 35
ER -
ID: 194816297