Reliability and variation in mitochondrial respiration in human adipose tissue
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Reliability and variation in mitochondrial respiration in human adipose tissue. / Sahl, Ronni Eg; Høy Helms, Eva Frederikke; Schmücker, Malte; Flensted-Jensen, Mathias; Ingersen, Arthur; Morville, Thomas; Dela, Flemming; Helge, Jørn Wulff; Larsen, Steen.
I: Adipocytes, Bind 10, Nr. 1, 2021, s. 605-611.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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TY - JOUR
T1 - Reliability and variation in mitochondrial respiration in human adipose tissue
AU - Sahl, Ronni Eg
AU - Høy Helms, Eva Frederikke
AU - Schmücker, Malte
AU - Flensted-Jensen, Mathias
AU - Ingersen, Arthur
AU - Morville, Thomas
AU - Dela, Flemming
AU - Helge, Jørn Wulff
AU - Larsen, Steen
N1 - Publisher Copyright: © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.
AB - Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.
KW - Adipose tissue
KW - coefficient of variation
KW - high resolution respirometry
KW - human
KW - mitochondria
U2 - 10.1080/21623945.2021.1991617
DO - 10.1080/21623945.2021.1991617
M3 - Journal article
C2 - 34709990
AN - SCOPUS:85120176224
VL - 10
SP - 605
EP - 611
JO - Adipocytes
JF - Adipocytes
SN - 1550-2082
IS - 1
ER -
ID: 286997015