Reliability and variation in mitochondrial respiration in human adipose tissue

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Standard

Reliability and variation in mitochondrial respiration in human adipose tissue. / Sahl, Ronni Eg; Høy Helms, Eva Frederikke; Schmücker, Malte; Flensted-Jensen, Mathias; Ingersen, Arthur; Morville, Thomas; Dela, Flemming; Helge, Jørn Wulff; Larsen, Steen.

I: Adipocytes, Bind 10, Nr. 1, 2021, s. 605-611.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt

Harvard

Sahl, RE, Høy Helms, EF, Schmücker, M, Flensted-Jensen, M, Ingersen, A, Morville, T, Dela, F, Helge, JW & Larsen, S 2021, 'Reliability and variation in mitochondrial respiration in human adipose tissue', Adipocytes, bind 10, nr. 1, s. 605-611. https://doi.org/10.1080/21623945.2021.1991617

APA

Sahl, R. E., Høy Helms, E. F., Schmücker, M., Flensted-Jensen, M., Ingersen, A., Morville, T., Dela, F., Helge, J. W., & Larsen, S. (2021). Reliability and variation in mitochondrial respiration in human adipose tissue. Adipocytes, 10(1), 605-611. https://doi.org/10.1080/21623945.2021.1991617

Vancouver

Sahl RE, Høy Helms EF, Schmücker M, Flensted-Jensen M, Ingersen A, Morville T o.a. Reliability and variation in mitochondrial respiration in human adipose tissue. Adipocytes. 2021;10(1):605-611. https://doi.org/10.1080/21623945.2021.1991617

Author

Sahl, Ronni Eg ; Høy Helms, Eva Frederikke ; Schmücker, Malte ; Flensted-Jensen, Mathias ; Ingersen, Arthur ; Morville, Thomas ; Dela, Flemming ; Helge, Jørn Wulff ; Larsen, Steen. / Reliability and variation in mitochondrial respiration in human adipose tissue. I: Adipocytes. 2021 ; Bind 10, Nr. 1. s. 605-611.

Bibtex

@article{deea3cb51b604e79a835eb34e5c79bce,

title = "Reliability and variation in mitochondrial respiration in human adipose tissue",

abstract = "Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.",

keywords = "Adipose tissue, coefficient of variation, high resolution respirometry, human, mitochondria",

author = "Sahl, {Ronni Eg} and {H{\o}y Helms}, {Eva Frederikke} and Malte Schm{\"u}cker and Mathias Flensted-Jensen and Arthur Ingersen and Thomas Morville and Flemming Dela and Helge, {J{\o}rn Wulff} and Steen Larsen",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",

year = "2021",

doi = "10.1080/21623945.2021.1991617",

language = "English",

volume = "10",

pages = "605--611",

journal = "Adipocytes",

issn = "1550-2082",

publisher = "New Century Health Publishers, LLC",

number = "1",

}

RIS

TY - JOUR

T1 - Reliability and variation in mitochondrial respiration in human adipose tissue

AU - Sahl, Ronni Eg

AU - Høy Helms, Eva Frederikke

AU - Schmücker, Malte

AU - Flensted-Jensen, Mathias

AU - Ingersen, Arthur

AU - Morville, Thomas

AU - Dela, Flemming

AU - Helge, Jørn Wulff

AU - Larsen, Steen

PY - 2021

Y1 - 2021

N2 - Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.

AB - Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.

KW - Adipose tissue

KW - coefficient of variation

KW - high resolution respirometry

KW - human

KW - mitochondria

U2 - 10.1080/21623945.2021.1991617

DO - 10.1080/21623945.2021.1991617

M3 - Journal article

C2 - 34709990

AN - SCOPUS:85120176224

VL - 10

SP - 605

EP - 611

JO - Adipocytes

JF - Adipocytes

SN - 1550-2082

IS - 1

ER -

ID: 286997015

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