Hepatic mitochondrial oxidative phosphorylation is normal in obese patients with and without type 2 diabetes
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Hepatic mitochondrial oxidative phosphorylation is normal in obese patients with and without type 2 diabetes. / Lund, Michael Taulo; Kristensen, Marianne Dalsgaard; Hansen, Merethe; Tveskov, Louise; Floyd, Andrea Karen; Støckel, Mikael; Vainer, Ben; Poulsen, Steen Seier; Helge, Jørn Wulff; Prats Gavalda, Clara; Dela, Flemming.
I: The Journal of Physiology, Bind 594, Nr. 15, 06.04.2016, s. 4351-4358.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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TY - JOUR
T1 - Hepatic mitochondrial oxidative phosphorylation is normal in obese patients with and without type 2 diabetes
AU - Lund, Michael Taulo
AU - Kristensen, Marianne Dalsgaard
AU - Hansen, Merethe
AU - Tveskov, Louise
AU - Floyd, Andrea Karen
AU - Støckel, Mikael
AU - Vainer, Ben
AU - Poulsen, Steen Seier
AU - Helge, Jørn Wulff
AU - Prats Gavalda, Clara
AU - Dela, Flemming
N1 - This article is protected by copyright. All rights reserved.
PY - 2016/4/6
Y1 - 2016/4/6
N2 - INTRODUCTION: Obese patients with (T2DM) and without (OB) type 2 diabetes are characterized by high hepatic lipid content and hepatic insulin resistance. This may be linked to impaired hepatic mitochondrial oxidative phosphorylation (OXPHOS) capacity. The aim of the present study was to investigate and compare hepatic mitochondrial OXPHOS capacity in T2DM, OB and non-obese controls (CON).METHODS: Seventeen obese patients (nine OB and eight T2DM) and six CON patients had perioperative liver biopsies taken. Samples were divided in three parts to measure 1: Complex I, II and IV linked respiration, 2: Citrate synthase (CS) activity and 3: Lipid droplet (LD) size and area (% of total tissue area filled by LDs).RESULTS: State 3 respiration of complex I, II and IV and the CS activity did not differ in OB, T2DM and CON. LD size was significantly higher in T2DM compared with CON and LD area tended (p = 0.10) to be higher in T2DM and OB compared with CON.CONCLUSION: The present findings indicate that hepatic OXPHOS capacity is not different in patients with markedly different weight and glycemic control. Furthermore, the results do not support impaired hepatic mitochondrial respiratory capacity playing a major role in the development of obesity-induced type 2 diabetes. This article is protected by copyright. All rights reserved.
AB - INTRODUCTION: Obese patients with (T2DM) and without (OB) type 2 diabetes are characterized by high hepatic lipid content and hepatic insulin resistance. This may be linked to impaired hepatic mitochondrial oxidative phosphorylation (OXPHOS) capacity. The aim of the present study was to investigate and compare hepatic mitochondrial OXPHOS capacity in T2DM, OB and non-obese controls (CON).METHODS: Seventeen obese patients (nine OB and eight T2DM) and six CON patients had perioperative liver biopsies taken. Samples were divided in three parts to measure 1: Complex I, II and IV linked respiration, 2: Citrate synthase (CS) activity and 3: Lipid droplet (LD) size and area (% of total tissue area filled by LDs).RESULTS: State 3 respiration of complex I, II and IV and the CS activity did not differ in OB, T2DM and CON. LD size was significantly higher in T2DM compared with CON and LD area tended (p = 0.10) to be higher in T2DM and OB compared with CON.CONCLUSION: The present findings indicate that hepatic OXPHOS capacity is not different in patients with markedly different weight and glycemic control. Furthermore, the results do not support impaired hepatic mitochondrial respiratory capacity playing a major role in the development of obesity-induced type 2 diabetes. This article is protected by copyright. All rights reserved.
U2 - 10.1113/JP272105
DO - 10.1113/JP272105
M3 - Journal article
C2 - 27060482
VL - 594
SP - 4351
EP - 4358
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - 15
ER -
ID: 160443775