Exercise in rats does not alter hypothalamic AMP-activated protein kinase activity
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Exercise in rats does not alter hypothalamic AMP-activated protein kinase activity. / Andersson, Ulrika; Treebak, Jonas Thue; Nielsen, Jakob Nis; Smith, Kirsty L.; Abbott, Caroline R.; Small, Caroline J.; Carling, David; Richter, Erik A.
I: Biochemical and Biophysical Research Communications, Bind 329, Nr. 2, 2005, s. 719-725.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Exercise in rats does not alter hypothalamic AMP-activated protein kinase activity
AU - Andersson, Ulrika
AU - Treebak, Jonas Thue
AU - Nielsen, Jakob Nis
AU - Smith, Kirsty L.
AU - Abbott, Caroline R.
AU - Small, Caroline J.
AU - Carling, David
AU - Richter, Erik A.
N1 - PUF 2005 5200 013
PY - 2005
Y1 - 2005
N2 - Recent studies have demonstrated that AMP-activated protein kinase (AMPK) in the hypothalamus is involved in the regulation of food intake. Because exercise is known to influence appetite and cause substrate depletion, it may also influence AMPK in the hypothalamus. Male rats that either rested or ran for 30 or 60 min on a treadmill (22 m/min, 10% slope) were sacrificed immediately after exercise or after 60 min recovery either in the fasted state or after oral gavage with glucose (3 g/kg body weight). Exercise decreased muscle and liver glycogen substantially. Hypothalamic total or a2-associated AMPK activity and phosphorylation state of the AMPK substrate acetyl-CoA carboxylase were not changed significantly immediately following treadmill running or during fed or fasted recovery. Plasma ghrelin increased (P < 0.05) by 40% during exercise whereas the concentration of PYY was unchanged. In recovery, glucose feeding increased plasma glucose and insulin concentrations whereas ghrelin and PYY decreased to (ghrelin) or below (PPY) resting levels. It is concluded that 1 h of strenuous exercise in rats does not elicit significant changes in hypothalamic AMPK activity despite an increase in plasma ghrelin. Thus, changes in energy metabolism during or after exercise are likely not coordinated by changes in hypothalamic AMPK activity.
AB - Recent studies have demonstrated that AMP-activated protein kinase (AMPK) in the hypothalamus is involved in the regulation of food intake. Because exercise is known to influence appetite and cause substrate depletion, it may also influence AMPK in the hypothalamus. Male rats that either rested or ran for 30 or 60 min on a treadmill (22 m/min, 10% slope) were sacrificed immediately after exercise or after 60 min recovery either in the fasted state or after oral gavage with glucose (3 g/kg body weight). Exercise decreased muscle and liver glycogen substantially. Hypothalamic total or a2-associated AMPK activity and phosphorylation state of the AMPK substrate acetyl-CoA carboxylase were not changed significantly immediately following treadmill running or during fed or fasted recovery. Plasma ghrelin increased (P < 0.05) by 40% during exercise whereas the concentration of PYY was unchanged. In recovery, glucose feeding increased plasma glucose and insulin concentrations whereas ghrelin and PYY decreased to (ghrelin) or below (PPY) resting levels. It is concluded that 1 h of strenuous exercise in rats does not elicit significant changes in hypothalamic AMPK activity despite an increase in plasma ghrelin. Thus, changes in energy metabolism during or after exercise are likely not coordinated by changes in hypothalamic AMPK activity.
U2 - 10.1016/j.bbrc.2005.02.027
DO - 10.1016/j.bbrc.2005.02.027
M3 - Journal article
C2 - 15737645
VL - 329
SP - 719
EP - 725
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -
ID: 91623