TY - JOUR

T1 - Downregulation of miR-125b in metastatic cutaneous malignant melanoma

AU - Glud, Martin

AU - Rossing, Maria

AU - Hother, Christoffer

AU - Holst, Line

AU - Hastrup, Nina

AU - Nielsen, Finn Cilius

AU - Gniadecki, Robert

AU - Drzewiecki, Krzysztof T

PY - 2010/12/1

Y1 - 2010/12/1

N2 - This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.

AB - This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.

U2 - http://dx.doi.org/10.1097/CMR.0b013e32833e32a1

DO - http://dx.doi.org/10.1097/CMR.0b013e32833e32a1

M3 - Journal article

VL - 20

SP - 479

EP - 484

JO - Melanoma Research

JF - Melanoma Research

SN - 0960-8931

IS - 6

ER -