Co-ordinated regulation of neurogenin-3 expression in the maternal and fetal pancreas during pregnancy
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Co-ordinated regulation of neurogenin-3 expression in the maternal and fetal pancreas during pregnancy. / Søstrup, Birgitte; Gaarn, Louise W; Nalla, Amarnadh; Billestrup, Nils; Nielsen, Jens H.
I: Acta Obstetricia et Gynecologica Scandinavica, Bind 93, Nr. 11, 11.2014, s. 1190-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Co-ordinated regulation of neurogenin-3 expression in the maternal and fetal pancreas during pregnancy
AU - Søstrup, Birgitte
AU - Gaarn, Louise W
AU - Nalla, Amarnadh
AU - Billestrup, Nils
AU - Nielsen, Jens H
N1 - © 2014 Nordic Federation of Societies of Obstetrics and Gynecology.
PY - 2014/11
Y1 - 2014/11
N2 - OBJECTIVE: Several studies have shown increased beta cell mass during pregnancy in both rodents and humans. Proliferation of existing beta cells seems to be the predominant mechanism in rodents, whereas the mechanism in humans is unclear. We hypothesized that neogenesis contributes to the increased beta cell mass in pregnancy and that circulating factors are involved.SAMPLES: Pancreatic tissue from mice and rat and serum from pregnant women.METHOD: Morphometric analysis of pancreas of pregnant and nonpregnant mice was carried out by immunocytochemical staining for the neogenic marker neurogenin-3. Messenger RNA levels of neurogenin-3 and the transcription factor musculoaponeurotic fibrosarcoma oncogene family protein B in fetal rat pancreas cells, cultured with serum from pregnant women, were measured by quantitative polymerase chain reaction.MAIN OUTCOME MEASURES: The number of neurogenin-3-positive cells present in pregnant mice was increased compared with nonpregnant mice. Neurogenin-3 and musculoaponeurotic fibrosarcoma oncogene family protein B mRNA was detected in fetal rat pancreas exposed to serum from pregnant women.RESULTS: In pregnant mice we found a 3.6-fold increase in beta cell volume at day 18 compared with nonpregnant mice and a 3.5-fold increase in neurogenin-3 volume at day 14, mainly located in the acinar compartment where it was eightfold higher than in nonpregnant mice. In fetal rat pancreatic cells exposed to serum from pregnant women we found a marked increase in both neurogenin-3 and musculoaponeurotic fibrosarcoma oncogene family protein B mRNA levels in fibroblast-like cells.CONCLUSION: These results suggest that neogenesis contributes to the increased beta cell mass in pregnancy and that circulating factors are involved in beta cell formation in both the maternal and fetal pancreas during pregnancy.
AB - OBJECTIVE: Several studies have shown increased beta cell mass during pregnancy in both rodents and humans. Proliferation of existing beta cells seems to be the predominant mechanism in rodents, whereas the mechanism in humans is unclear. We hypothesized that neogenesis contributes to the increased beta cell mass in pregnancy and that circulating factors are involved.SAMPLES: Pancreatic tissue from mice and rat and serum from pregnant women.METHOD: Morphometric analysis of pancreas of pregnant and nonpregnant mice was carried out by immunocytochemical staining for the neogenic marker neurogenin-3. Messenger RNA levels of neurogenin-3 and the transcription factor musculoaponeurotic fibrosarcoma oncogene family protein B in fetal rat pancreas cells, cultured with serum from pregnant women, were measured by quantitative polymerase chain reaction.MAIN OUTCOME MEASURES: The number of neurogenin-3-positive cells present in pregnant mice was increased compared with nonpregnant mice. Neurogenin-3 and musculoaponeurotic fibrosarcoma oncogene family protein B mRNA was detected in fetal rat pancreas exposed to serum from pregnant women.RESULTS: In pregnant mice we found a 3.6-fold increase in beta cell volume at day 18 compared with nonpregnant mice and a 3.5-fold increase in neurogenin-3 volume at day 14, mainly located in the acinar compartment where it was eightfold higher than in nonpregnant mice. In fetal rat pancreatic cells exposed to serum from pregnant women we found a marked increase in both neurogenin-3 and musculoaponeurotic fibrosarcoma oncogene family protein B mRNA levels in fibroblast-like cells.CONCLUSION: These results suggest that neogenesis contributes to the increased beta cell mass in pregnancy and that circulating factors are involved in beta cell formation in both the maternal and fetal pancreas during pregnancy.
KW - Animals
KW - Basic Helix-Loop-Helix Transcription Factors
KW - Female
KW - Fetus
KW - Humans
KW - Insulin-Secreting Cells
KW - Mice
KW - Nerve Tissue Proteins
KW - Pancreas
KW - Pregnancy
KW - RNA, Messenger
KW - Rats
KW - Reverse Transcriptase Polymerase Chain Reaction
U2 - 10.1111/aogs.12495
DO - 10.1111/aogs.12495
M3 - Journal article
C2 - 25179808
VL - 93
SP - 1190
EP - 1197
JO - Acta Obstetricia et Gynecologica Scandinavica
JF - Acta Obstetricia et Gynecologica Scandinavica
SN - 0001-6349
IS - 11
ER -
ID: 132899601