Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.

TitelMalaria Immunology : Targeting the Surface of Infected Erythrocytes
Antal sider10
ForlagHumana Press
ISBN (Trykt)978-1-0716-2188-2
ISBN (Elektronisk)978-1-0716-2189-9
StatusUdgivet - 2022
NavnMethods in molecular biology (Clifton, N.J.)

Bibliografisk note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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