Whole-genome shotgun sequencing of mitochondria from ancient hair shafts.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Lynn P Tomsho
  • Snjezana Rendulic
  • Michael Packard
  • Daniela I Drautz
  • Andrei Sher
  • Alexei Tikhonov
  • Love Dalén
  • Tatyana Kuznetsova
  • Pavel Kosintsev
  • Paula F Campos
  • Thomas Higham
  • Matthew J Collins
  • Andrew S Wilson
  • Fyodor Shidlovskiy
  • Bernard Buigues
  • Mietje Germonpré
  • Anders Götherström
  • Paola Iacumin
  • Vladimir Nikolaev
  • Malgosia Nowak-Kemp
  • James R Knight
  • Gerard P Irzyk
  • Clotilde S Perbost
  • Karin M Fredrikson
  • Timothy T Harkins
  • Sharon Sheridan
  • Webb Miller
  • Stephan C Schuster
Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.
Udgivelsesdato: 2007-Sep-28
Udgave nummer5846
Sider (fra-til)1927-30
Antal sider3
StatusUdgivet - 2007

Bibliografisk note

Keywords: Animals; Bone and Bones; DNA Damage; DNA, Mitochondrial; Elephants; Genes, Mitochondrial; Genome; Hair; History, Ancient; Mitochondria; Molecular Sequence Data; Preservation, Biological; Sequence Analysis, DNA; Siberia; Temperature

ID: 3848417