Standard
Whole-genome shotgun sequencing of mitochondria from ancient hair shafts. / Gilbert, M Thomas P; Tomsho, Lynn P; Rendulic, Snjezana; Packard, Michael; Drautz, Daniela I; Sher, Andrei; Tikhonov, Alexei; Dalén, Love; Kuznetsova, Tatyana; Kosintsev, Pavel; Campos, Paula F; Higham, Thomas; Collins, Matthew J; Wilson, Andrew S; Shidlovskiy, Fyodor; Buigues, Bernard; Ericson, Per G P; Germonpré, Mietje; Götherström, Anders; Iacumin, Paola; Nikolaev, Vladimir; Nowak-Kemp, Malgosia; Willerslev, Eske; Knight, James R; Irzyk, Gerard P; Perbost, Clotilde S; Fredrikson, Karin M; Harkins, Timothy T; Sheridan, Sharon; Miller, Webb; Schuster, Stephan C.
I:
Science, Bind 317, Nr. 5846, 2007, s. 1927-30.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
Gilbert, MTP, Tomsho, LP, Rendulic, S, Packard, M, Drautz, DI, Sher, A, Tikhonov, A, Dalén, L, Kuznetsova, T, Kosintsev, P, Campos, PF, Higham, T, Collins, MJ, Wilson, AS, Shidlovskiy, F, Buigues, B
, Ericson, PGP, Germonpré, M, Götherström, A, Iacumin, P, Nikolaev, V, Nowak-Kemp, M
, Willerslev, E, Knight, JR, Irzyk, GP, Perbost, CS, Fredrikson, KM, Harkins, TT, Sheridan, S, Miller, W & Schuster, SC 2007, '
Whole-genome shotgun sequencing of mitochondria from ancient hair shafts.',
Science, bind 317, nr. 5846, s. 1927-30.
https://doi.org/10.1126/science.1146971
APA
Gilbert, M. T. P., Tomsho, L. P., Rendulic, S., Packard, M., Drautz, D. I., Sher, A., Tikhonov, A., Dalén, L., Kuznetsova, T., Kosintsev, P., Campos, P. F., Higham, T., Collins, M. J., Wilson, A. S., Shidlovskiy, F., Buigues, B.
, Ericson, P. G. P., Germonpré, M., Götherström, A., ... Schuster, S. C. (2007).
Whole-genome shotgun sequencing of mitochondria from ancient hair shafts. Science,
317(5846), 1927-30.
https://doi.org/10.1126/science.1146971
Vancouver
Gilbert MTP, Tomsho LP, Rendulic S, Packard M, Drautz DI, Sher A o.a.
Whole-genome shotgun sequencing of mitochondria from ancient hair shafts. Science. 2007;317(5846):1927-30.
https://doi.org/10.1126/science.1146971
Author
Gilbert, M Thomas P ; Tomsho, Lynn P ; Rendulic, Snjezana ; Packard, Michael ; Drautz, Daniela I ; Sher, Andrei ; Tikhonov, Alexei ; Dalén, Love ; Kuznetsova, Tatyana ; Kosintsev, Pavel ; Campos, Paula F ; Higham, Thomas ; Collins, Matthew J ; Wilson, Andrew S ; Shidlovskiy, Fyodor ; Buigues, Bernard ; Ericson, Per G P ; Germonpré, Mietje ; Götherström, Anders ; Iacumin, Paola ; Nikolaev, Vladimir ; Nowak-Kemp, Malgosia ; Willerslev, Eske ; Knight, James R ; Irzyk, Gerard P ; Perbost, Clotilde S ; Fredrikson, Karin M ; Harkins, Timothy T ; Sheridan, Sharon ; Miller, Webb ; Schuster, Stephan C. / Whole-genome shotgun sequencing of mitochondria from ancient hair shafts. I: Science. 2007 ; Bind 317, Nr. 5846. s. 1927-30.
Bibtex
@article{8e246c90149411ddbee902004c4f4f50,
title = "Whole-genome shotgun sequencing of mitochondria from ancient hair shafts.",
abstract = "Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.",
author = "Gilbert, {M Thomas P} and Tomsho, {Lynn P} and Snjezana Rendulic and Michael Packard and Drautz, {Daniela I} and Andrei Sher and Alexei Tikhonov and Love Dal{\'e}n and Tatyana Kuznetsova and Pavel Kosintsev and Campos, {Paula F} and Thomas Higham and Collins, {Matthew J} and Wilson, {Andrew S} and Fyodor Shidlovskiy and Bernard Buigues and Ericson, {Per G P} and Mietje Germonpr{\'e} and Anders G{\"o}therstr{\"o}m and Paola Iacumin and Vladimir Nikolaev and Malgosia Nowak-Kemp and Eske Willerslev and Knight, {James R} and Irzyk, {Gerard P} and Perbost, {Clotilde S} and Fredrikson, {Karin M} and Harkins, {Timothy T} and Sharon Sheridan and Webb Miller and Schuster, {Stephan C}",
note = "Keywords: Animals; Bone and Bones; DNA Damage; DNA, Mitochondrial; Elephants; Genes, Mitochondrial; Genome; Hair; History, Ancient; Mitochondria; Molecular Sequence Data; Preservation, Biological; Sequence Analysis, DNA; Siberia; Temperature",
year = "2007",
doi = "10.1126/science.1146971",
language = "English",
volume = "317",
pages = "1927--30",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5846",
}
RIS
TY - JOUR
T1 - Whole-genome shotgun sequencing of mitochondria from ancient hair shafts.
AU - Gilbert, M Thomas P
AU - Tomsho, Lynn P
AU - Rendulic, Snjezana
AU - Packard, Michael
AU - Drautz, Daniela I
AU - Sher, Andrei
AU - Tikhonov, Alexei
AU - Dalén, Love
AU - Kuznetsova, Tatyana
AU - Kosintsev, Pavel
AU - Campos, Paula F
AU - Higham, Thomas
AU - Collins, Matthew J
AU - Wilson, Andrew S
AU - Shidlovskiy, Fyodor
AU - Buigues, Bernard
AU - Ericson, Per G P
AU - Germonpré, Mietje
AU - Götherström, Anders
AU - Iacumin, Paola
AU - Nikolaev, Vladimir
AU - Nowak-Kemp, Malgosia
AU - Willerslev, Eske
AU - Knight, James R
AU - Irzyk, Gerard P
AU - Perbost, Clotilde S
AU - Fredrikson, Karin M
AU - Harkins, Timothy T
AU - Sheridan, Sharon
AU - Miller, Webb
AU - Schuster, Stephan C
N1 - Keywords: Animals; Bone and Bones; DNA Damage; DNA, Mitochondrial; Elephants; Genes, Mitochondrial; Genome; Hair; History, Ancient; Mitochondria; Molecular Sequence Data; Preservation, Biological; Sequence Analysis, DNA; Siberia; Temperature
PY - 2007
Y1 - 2007
N2 - Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.
AB - Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.
U2 - 10.1126/science.1146971
DO - 10.1126/science.1146971
M3 - Journal article
C2 - 17901335
VL - 317
SP - 1927
EP - 1930
JO - Science
JF - Science
SN - 0036-8075
IS - 5846
ER -
