Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone.

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Standard

Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone. / Jackerott, Malene; Lee, Ying C; Møllgård, Kjeld; Kofod, Hans; Jensen, Janne; Rohleder, Sonja; Neubauer, Nicole; Gaarn, Louise W; Lykke, Jeanette; Dodge, Rikke; Dalgaard, Louise T; Søstrup, Birgitte; Jensen, Dennis B; Thim, Lars; Nexø, Ebba; Thams, Peter; Bisgaard, Hanne Cathrine; Nielsen, Jens H.

I: Endocrinology, Bind 147, Nr. 12, 2006, s. 5752-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Jackerott, M, Lee, YC, Møllgård, K, Kofod, H, Jensen, J, Rohleder, S, Neubauer, N, Gaarn, LW, Lykke, J, Dodge, R, Dalgaard, LT, Søstrup, B, Jensen, DB, Thim, L, Nexø, E, Thams, P, Bisgaard, HC & Nielsen, JH 2006, 'Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone.', Endocrinology, bind 147, nr. 12, s. 5752-9. https://doi.org/10.1210/en.2006-0601

APA

Jackerott, M., Lee, Y. C., Møllgård, K., Kofod, H., Jensen, J., Rohleder, S., ... Nielsen, J. H. (2006). Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone. Endocrinology, 147(12), 5752-9. https://doi.org/10.1210/en.2006-0601

Vancouver

Jackerott M, Lee YC, Møllgård K, Kofod H, Jensen J, Rohleder S o.a. Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone. Endocrinology. 2006;147(12):5752-9. https://doi.org/10.1210/en.2006-0601

Author

Jackerott, Malene ; Lee, Ying C ; Møllgård, Kjeld ; Kofod, Hans ; Jensen, Janne ; Rohleder, Sonja ; Neubauer, Nicole ; Gaarn, Louise W ; Lykke, Jeanette ; Dodge, Rikke ; Dalgaard, Louise T ; Søstrup, Birgitte ; Jensen, Dennis B ; Thim, Lars ; Nexø, Ebba ; Thams, Peter ; Bisgaard, Hanne Cathrine ; Nielsen, Jens H. / Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone. I: Endocrinology. 2006 ; Bind 147, Nr. 12. s. 5752-9.

Bibtex

@article{2d774bb0ac1011ddb5e9000ea68e967b,
title = "Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone.",
abstract = "Trefoil factors (TFFs) 1, 2, and 3 are expressed in mucosal epithelia. TFFs are particular abundant in the intestine in which they play a crucial role in maintenance and restitution of the epithelium. Because pancreas developmentally arises from the primitive foregut, we explored the expression of TFFs in the pancreas in man and rat. Immunocytochemical staining of adult human pancreas showed abundant TFF3 immunoreactivity in pancreatic islets and some duct cells, whereas weak TFF1 and no TFF2 staining were detected. In the islets TFF3 localized to most insulin and some glucagon and pancreatic polypeptide-producing cells. TFF3 immunoreactivity was colocalized with insulin and glucagon in distinct cell clusters in human fetal pancreas at wk 14 and in the newborn rat pancreas. In isolated human and rat islets, TFF3 and TFF1 mRNA was identified by RT-PCR, and TFF3 protein was detected in human pancreas and islets by ELISA. Exposure of neonatal rat islets or insulinoma cells to GH, a known beta-cell growth factor, resulted in markedly increased TFF3 but decreased TFF1 mRNA levels. The effect of GH on TFF3 expression was confirmed by Western blot. Culture of neonatal rat islets in the presence of TFF3 resulted in attachment and migration of the islet cells, but no effects on proliferation, insulin secretion or cytokine-induced apoptosis were seen. These data demonstrate expression of TFFs in the endocrine pancreas, but their possible functions remain unknown.",
author = "Malene Jackerott and Lee, {Ying C} and Kjeld M{\o}llg{\aa}rd and Hans Kofod and Janne Jensen and Sonja Rohleder and Nicole Neubauer and Gaarn, {Louise W} and Jeanette Lykke and Rikke Dodge and Dalgaard, {Louise T} and Birgitte S{\o}strup and Jensen, {Dennis B} and Lars Thim and Ebba Nex{\o} and Peter Thams and Bisgaard, {Hanne Cathrine} and Nielsen, {Jens H}",
note = "Keywords: Adult; Animals; Animals, Newborn; Apoptosis; Cell Movement; Cell Proliferation; Gene Expression Regulation; Growth Hormone; Humans; Insulin; Insulinoma; Islets of Langerhans; Pancreatic Neoplasms; Peptides; Rats; Tissue Distribution; Tumor Cells, Cultured",
year = "2006",
doi = "10.1210/en.2006-0601",
language = "English",
volume = "147",
pages = "5752--9",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "12",

}

RIS

TY - JOUR

T1 - Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone.

AU - Jackerott, Malene

AU - Lee, Ying C

AU - Møllgård, Kjeld

AU - Kofod, Hans

AU - Jensen, Janne

AU - Rohleder, Sonja

AU - Neubauer, Nicole

AU - Gaarn, Louise W

AU - Lykke, Jeanette

AU - Dodge, Rikke

AU - Dalgaard, Louise T

AU - Søstrup, Birgitte

AU - Jensen, Dennis B

AU - Thim, Lars

AU - Nexø, Ebba

AU - Thams, Peter

AU - Bisgaard, Hanne Cathrine

AU - Nielsen, Jens H

N1 - Keywords: Adult; Animals; Animals, Newborn; Apoptosis; Cell Movement; Cell Proliferation; Gene Expression Regulation; Growth Hormone; Humans; Insulin; Insulinoma; Islets of Langerhans; Pancreatic Neoplasms; Peptides; Rats; Tissue Distribution; Tumor Cells, Cultured

PY - 2006

Y1 - 2006

N2 - Trefoil factors (TFFs) 1, 2, and 3 are expressed in mucosal epithelia. TFFs are particular abundant in the intestine in which they play a crucial role in maintenance and restitution of the epithelium. Because pancreas developmentally arises from the primitive foregut, we explored the expression of TFFs in the pancreas in man and rat. Immunocytochemical staining of adult human pancreas showed abundant TFF3 immunoreactivity in pancreatic islets and some duct cells, whereas weak TFF1 and no TFF2 staining were detected. In the islets TFF3 localized to most insulin and some glucagon and pancreatic polypeptide-producing cells. TFF3 immunoreactivity was colocalized with insulin and glucagon in distinct cell clusters in human fetal pancreas at wk 14 and in the newborn rat pancreas. In isolated human and rat islets, TFF3 and TFF1 mRNA was identified by RT-PCR, and TFF3 protein was detected in human pancreas and islets by ELISA. Exposure of neonatal rat islets or insulinoma cells to GH, a known beta-cell growth factor, resulted in markedly increased TFF3 but decreased TFF1 mRNA levels. The effect of GH on TFF3 expression was confirmed by Western blot. Culture of neonatal rat islets in the presence of TFF3 resulted in attachment and migration of the islet cells, but no effects on proliferation, insulin secretion or cytokine-induced apoptosis were seen. These data demonstrate expression of TFFs in the endocrine pancreas, but their possible functions remain unknown.

AB - Trefoil factors (TFFs) 1, 2, and 3 are expressed in mucosal epithelia. TFFs are particular abundant in the intestine in which they play a crucial role in maintenance and restitution of the epithelium. Because pancreas developmentally arises from the primitive foregut, we explored the expression of TFFs in the pancreas in man and rat. Immunocytochemical staining of adult human pancreas showed abundant TFF3 immunoreactivity in pancreatic islets and some duct cells, whereas weak TFF1 and no TFF2 staining were detected. In the islets TFF3 localized to most insulin and some glucagon and pancreatic polypeptide-producing cells. TFF3 immunoreactivity was colocalized with insulin and glucagon in distinct cell clusters in human fetal pancreas at wk 14 and in the newborn rat pancreas. In isolated human and rat islets, TFF3 and TFF1 mRNA was identified by RT-PCR, and TFF3 protein was detected in human pancreas and islets by ELISA. Exposure of neonatal rat islets or insulinoma cells to GH, a known beta-cell growth factor, resulted in markedly increased TFF3 but decreased TFF1 mRNA levels. The effect of GH on TFF3 expression was confirmed by Western blot. Culture of neonatal rat islets in the presence of TFF3 resulted in attachment and migration of the islet cells, but no effects on proliferation, insulin secretion or cytokine-induced apoptosis were seen. These data demonstrate expression of TFFs in the endocrine pancreas, but their possible functions remain unknown.

U2 - 10.1210/en.2006-0601

DO - 10.1210/en.2006-0601

M3 - Journal article

C2 - 16973727

VL - 147

SP - 5752

EP - 5759

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 12

ER -

ID: 8443685