Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark

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Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark. / Pinna, Nishal Kumar; Anjana, Ranjit Mohan; Saxena, Shruti; Dutta, Anirban; Gnanaprakash, Visvanathan; Rameshkumar, Gnanavadivel; Aswath, Sukumaran; Raghavan, Srividhya; Rani, Coimbatore Subramanian Shanthi; Radha, Venkatesan; Balasubramanyam, Muthuswamy; Pant, Archana; Nielsen, Trine; Jørgensen, Torben; Færch, Kristine; Kashani, Alireza; Silva, Maria Camila Alvarez; Vestergaard, Henrik; Hansen, Tue Haldor; Hansen, Torben; Arumugam, Manimozhiyan; Nair, Gopinath Balakrish; Das, Bhabatosh; Pedersen, Oluf; Mohan, Viswanathan; Mande, Sharmila Shekhar.

I: Genome Medicine, Bind 13, 36, 2021.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Pinna, NK, Anjana, RM, Saxena, S, Dutta, A, Gnanaprakash, V, Rameshkumar, G, Aswath, S, Raghavan, S, Rani, CSS, Radha, V, Balasubramanyam, M, Pant, A, Nielsen, T, Jørgensen, T, Færch, K, Kashani, A, Silva, MCA, Vestergaard, H, Hansen, TH, Hansen, T, Arumugam, M, Nair, GB, Das, B, Pedersen, O, Mohan, V & Mande, SS 2021, 'Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark', Genome Medicine, bind 13, 36. https://doi.org/10.1186/s13073-021-00851-9

APA

Pinna, N. K., Anjana, R. M., Saxena, S., Dutta, A., Gnanaprakash, V., Rameshkumar, G., Aswath, S., Raghavan, S., Rani, C. S. S., Radha, V., Balasubramanyam, M., Pant, A., Nielsen, T., Jørgensen, T., Færch, K., Kashani, A., Silva, M. C. A., Vestergaard, H., Hansen, T. H., ... Mande, S. S. (2021). Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark. Genome Medicine, 13, [36]. https://doi.org/10.1186/s13073-021-00851-9

Vancouver

Pinna NK, Anjana RM, Saxena S, Dutta A, Gnanaprakash V, Rameshkumar G o.a. Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark. Genome Medicine. 2021;13. 36. https://doi.org/10.1186/s13073-021-00851-9

Author

Pinna, Nishal Kumar ; Anjana, Ranjit Mohan ; Saxena, Shruti ; Dutta, Anirban ; Gnanaprakash, Visvanathan ; Rameshkumar, Gnanavadivel ; Aswath, Sukumaran ; Raghavan, Srividhya ; Rani, Coimbatore Subramanian Shanthi ; Radha, Venkatesan ; Balasubramanyam, Muthuswamy ; Pant, Archana ; Nielsen, Trine ; Jørgensen, Torben ; Færch, Kristine ; Kashani, Alireza ; Silva, Maria Camila Alvarez ; Vestergaard, Henrik ; Hansen, Tue Haldor ; Hansen, Torben ; Arumugam, Manimozhiyan ; Nair, Gopinath Balakrish ; Das, Bhabatosh ; Pedersen, Oluf ; Mohan, Viswanathan ; Mande, Sharmila Shekhar. / Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark. I: Genome Medicine. 2021 ; Bind 13.

Bibtex

@article{431ffd6d325b4270920861465613b8ad,
title = "Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark",
abstract = "Background: Recent studies have indicated an association of gut microbiota and microbial metabolites with type 2 diabetes mellitus (T2D). However, large-scale investigation of the gut microbiota of “prediabetic” (PD) subjects has not been reported. Identifying robust gut microbiome signatures of prediabetes and characterizing early prediabetic stages is important for the understanding of disease development and could be crucial in early diagnosis and prevention. Methods: The current study performed amplification and sequencing on the variable regions (V1–V5) of the 16S rRNA genes to profile and compare gut microbiota of prediabetic individuals (N = 262) with normoglycemic individuals (N = 275) from two cohorts in India and Denmark. Similarly, fasting serum inflammatory biomarkers were profiled from the study participants. Results: After correcting for strong country-specific cohort effect, 16 operational taxonomic units (OTUs) including members from the genera Prevotella9, Phascolarctobacterium, Barnesiella, Flavonifractor, Tyzzerella_4, Bacteroides, Faecalibacterium, and Agathobacter were identified as enriched in normoglycaemic subjects with respect to the subjects with prediabetes using a negative binomial Wald test. We also identified 144 OTUs enriched in the prediabetic subjects, which included members from the genera Megasphaera, Streptococcus, Prevotella9, Alistipes, Mitsuokella, Escherichia/Shigella, Prevotella2, Vibrio, Lactobacillus, Alloprevotella, Rhodococcus, and Klebsiella. Comparative analyses of relative abundance of bacterial taxa revealed that the Streptococcus, Escherichia/Shigella, Prevotella2, Vibrio, and Alloprevotella OTUs exhibited more than fourfold enrichment in the gut microbiota of prediabetic subjects. When considering subjects from the two geographies separately, we were able to identify additional gut microbiome signatures of prediabetes. The study reports a probable association of Megasphaera OTU(s) with impaired glucose tolerance, which is significantly pronounced in Indian subjects. While the overall results confirm a state of proinflammation as early as in prediabetes, the Indian cohort exhibited a characteristic pattern of abundance of inflammatory markers indicating low-grade intestinal inflammation at an overall population level, irrespective of glycemic status. Conclusions: The results present trans-ethnic gut microbiome and inflammation signatures associated with prediabetes, in Indian and Danish populations. The identified associations may be explored further as potential early indicators for individuals at risk of dysglycemia.",
author = "Pinna, {Nishal Kumar} and Anjana, {Ranjit Mohan} and Shruti Saxena and Anirban Dutta and Visvanathan Gnanaprakash and Gnanavadivel Rameshkumar and Sukumaran Aswath and Srividhya Raghavan and Rani, {Coimbatore Subramanian Shanthi} and Venkatesan Radha and Muthuswamy Balasubramanyam and Archana Pant and Trine Nielsen and Torben J{\o}rgensen and Kristine F{\ae}rch and Alireza Kashani and Silva, {Maria Camila Alvarez} and Henrik Vestergaard and Hansen, {Tue Haldor} and Torben Hansen and Manimozhiyan Arumugam and Nair, {Gopinath Balakrish} and Bhabatosh Das and Oluf Pedersen and Viswanathan Mohan and Mande, {Sharmila Shekhar}",
year = "2021",
doi = "10.1186/s13073-021-00851-9",
language = "English",
volume = "13",
journal = "Genome Medicine",
issn = "1756-994X",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark

AU - Pinna, Nishal Kumar

AU - Anjana, Ranjit Mohan

AU - Saxena, Shruti

AU - Dutta, Anirban

AU - Gnanaprakash, Visvanathan

AU - Rameshkumar, Gnanavadivel

AU - Aswath, Sukumaran

AU - Raghavan, Srividhya

AU - Rani, Coimbatore Subramanian Shanthi

AU - Radha, Venkatesan

AU - Balasubramanyam, Muthuswamy

AU - Pant, Archana

AU - Nielsen, Trine

AU - Jørgensen, Torben

AU - Færch, Kristine

AU - Kashani, Alireza

AU - Silva, Maria Camila Alvarez

AU - Vestergaard, Henrik

AU - Hansen, Tue Haldor

AU - Hansen, Torben

AU - Arumugam, Manimozhiyan

AU - Nair, Gopinath Balakrish

AU - Das, Bhabatosh

AU - Pedersen, Oluf

AU - Mohan, Viswanathan

AU - Mande, Sharmila Shekhar

PY - 2021

Y1 - 2021

N2 - Background: Recent studies have indicated an association of gut microbiota and microbial metabolites with type 2 diabetes mellitus (T2D). However, large-scale investigation of the gut microbiota of “prediabetic” (PD) subjects has not been reported. Identifying robust gut microbiome signatures of prediabetes and characterizing early prediabetic stages is important for the understanding of disease development and could be crucial in early diagnosis and prevention. Methods: The current study performed amplification and sequencing on the variable regions (V1–V5) of the 16S rRNA genes to profile and compare gut microbiota of prediabetic individuals (N = 262) with normoglycemic individuals (N = 275) from two cohorts in India and Denmark. Similarly, fasting serum inflammatory biomarkers were profiled from the study participants. Results: After correcting for strong country-specific cohort effect, 16 operational taxonomic units (OTUs) including members from the genera Prevotella9, Phascolarctobacterium, Barnesiella, Flavonifractor, Tyzzerella_4, Bacteroides, Faecalibacterium, and Agathobacter were identified as enriched in normoglycaemic subjects with respect to the subjects with prediabetes using a negative binomial Wald test. We also identified 144 OTUs enriched in the prediabetic subjects, which included members from the genera Megasphaera, Streptococcus, Prevotella9, Alistipes, Mitsuokella, Escherichia/Shigella, Prevotella2, Vibrio, Lactobacillus, Alloprevotella, Rhodococcus, and Klebsiella. Comparative analyses of relative abundance of bacterial taxa revealed that the Streptococcus, Escherichia/Shigella, Prevotella2, Vibrio, and Alloprevotella OTUs exhibited more than fourfold enrichment in the gut microbiota of prediabetic subjects. When considering subjects from the two geographies separately, we were able to identify additional gut microbiome signatures of prediabetes. The study reports a probable association of Megasphaera OTU(s) with impaired glucose tolerance, which is significantly pronounced in Indian subjects. While the overall results confirm a state of proinflammation as early as in prediabetes, the Indian cohort exhibited a characteristic pattern of abundance of inflammatory markers indicating low-grade intestinal inflammation at an overall population level, irrespective of glycemic status. Conclusions: The results present trans-ethnic gut microbiome and inflammation signatures associated with prediabetes, in Indian and Danish populations. The identified associations may be explored further as potential early indicators for individuals at risk of dysglycemia.

AB - Background: Recent studies have indicated an association of gut microbiota and microbial metabolites with type 2 diabetes mellitus (T2D). However, large-scale investigation of the gut microbiota of “prediabetic” (PD) subjects has not been reported. Identifying robust gut microbiome signatures of prediabetes and characterizing early prediabetic stages is important for the understanding of disease development and could be crucial in early diagnosis and prevention. Methods: The current study performed amplification and sequencing on the variable regions (V1–V5) of the 16S rRNA genes to profile and compare gut microbiota of prediabetic individuals (N = 262) with normoglycemic individuals (N = 275) from two cohorts in India and Denmark. Similarly, fasting serum inflammatory biomarkers were profiled from the study participants. Results: After correcting for strong country-specific cohort effect, 16 operational taxonomic units (OTUs) including members from the genera Prevotella9, Phascolarctobacterium, Barnesiella, Flavonifractor, Tyzzerella_4, Bacteroides, Faecalibacterium, and Agathobacter were identified as enriched in normoglycaemic subjects with respect to the subjects with prediabetes using a negative binomial Wald test. We also identified 144 OTUs enriched in the prediabetic subjects, which included members from the genera Megasphaera, Streptococcus, Prevotella9, Alistipes, Mitsuokella, Escherichia/Shigella, Prevotella2, Vibrio, Lactobacillus, Alloprevotella, Rhodococcus, and Klebsiella. Comparative analyses of relative abundance of bacterial taxa revealed that the Streptococcus, Escherichia/Shigella, Prevotella2, Vibrio, and Alloprevotella OTUs exhibited more than fourfold enrichment in the gut microbiota of prediabetic subjects. When considering subjects from the two geographies separately, we were able to identify additional gut microbiome signatures of prediabetes. The study reports a probable association of Megasphaera OTU(s) with impaired glucose tolerance, which is significantly pronounced in Indian subjects. While the overall results confirm a state of proinflammation as early as in prediabetes, the Indian cohort exhibited a characteristic pattern of abundance of inflammatory markers indicating low-grade intestinal inflammation at an overall population level, irrespective of glycemic status. Conclusions: The results present trans-ethnic gut microbiome and inflammation signatures associated with prediabetes, in Indian and Danish populations. The identified associations may be explored further as potential early indicators for individuals at risk of dysglycemia.

U2 - 10.1186/s13073-021-00851-9

DO - 10.1186/s13073-021-00851-9

M3 - Journal article

C2 - 33658065

AN - SCOPUS:85101996661

VL - 13

JO - Genome Medicine

JF - Genome Medicine

SN - 1756-994X

M1 - 36

ER -

ID: 259050625