Training state and skeletal muscle autophagy in response to 36 h of fasting

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Training state and skeletal muscle autophagy in response to 36 h of fasting. / Dethlefsen, Maja Munk; Bertholdt, Lærke; Gudiksen, Anders; Stankiewicz, Tomasz; Bangsbo, Jens; van Hall, Gerrit; Plomgaard, Peter; Pilegaard, Henriette.

I: Journal of Applied Physiology, Bind 125, Nr. 5, 11.2018, s. 1609-1619.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Dethlefsen, MM, Bertholdt, L, Gudiksen, A, Stankiewicz, T, Bangsbo, J, van Hall, G, Plomgaard, P & Pilegaard, H 2018, 'Training state and skeletal muscle autophagy in response to 36 h of fasting', Journal of Applied Physiology, bind 125, nr. 5, s. 1609-1619. https://doi.org/10.1152/japplphysiol.01146.2017

APA

Dethlefsen, M. M., Bertholdt, L., Gudiksen, A., Stankiewicz, T., Bangsbo, J., van Hall, G., Plomgaard, P., & Pilegaard, H. (2018). Training state and skeletal muscle autophagy in response to 36 h of fasting. Journal of Applied Physiology, 125(5), 1609-1619. https://doi.org/10.1152/japplphysiol.01146.2017

Vancouver

Dethlefsen MM, Bertholdt L, Gudiksen A, Stankiewicz T, Bangsbo J, van Hall G o.a. Training state and skeletal muscle autophagy in response to 36 h of fasting. Journal of Applied Physiology. 2018 nov;125(5):1609-1619. https://doi.org/10.1152/japplphysiol.01146.2017

Author

Dethlefsen, Maja Munk ; Bertholdt, Lærke ; Gudiksen, Anders ; Stankiewicz, Tomasz ; Bangsbo, Jens ; van Hall, Gerrit ; Plomgaard, Peter ; Pilegaard, Henriette. / Training state and skeletal muscle autophagy in response to 36 h of fasting. I: Journal of Applied Physiology. 2018 ; Bind 125, Nr. 5. s. 1609-1619.

Bibtex

@article{85d8e9b139c746ffa3a90f21d385ce08,
title = "Training state and skeletal muscle autophagy in response to 36 h of fasting",
abstract = "The present study aimed at investigating fasting-induced responses in regulators and markers of autophagy in vastus lateralis muscle from untrained and trained human subjects. Untrained and trained subjects (based on maximum oxygen uptake, muscle citrate synthase activity, and oxidative phosphorylation protein level) fasted for 36 h with vastus lateralis muscle biopsies obtained at 2, 12, 24, and 36 h after a standardized meal. Fasting reduced (P 0.05) skeletal muscle microtubule-associated protein-1A/1B light chain 3 (LC3)I, LC3II, and adaptor protein sequestosome 1/p62 protein content in untrained subjects only. Moreover, skeletal muscle RAC-alpha serine/threonine-protein kinase (AKT) Thr308, AMP-activated protein kinase (AMPK) Thr172, and Unc-51-like autophagy-activating kinase-1 (ULK1) Ser555 phosphorylation state, as well as Bcl-2-interacting coiled-coil protein-1 (Beclin1) and ULK1 Ser757 phosphorylation, was lower (P 0.05) in trained than untrained subjects during fasting. In addition, the plasma concentrations of several amino acids were higher (P 0.05) in trained than untrained subjects, and the plasma concentration profile of several amino acids was different in untrained and trained subjects during fasting. Taken together, these findings suggest that 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle and that training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle. NEW & NOTEWORTHY This study showed that skeletal muscle autophagy was only modestly affected in humans by 36 h of fasting. Hence, 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle, and training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle. ",
keywords = "Faculty of Science, Fasting, Exercise training, Skeletal muscle, Autophagy, Signaling, Plasma amino acid, Autophagy, Exercise training, Fasting, Plasma amino acid, Signaling, Skeletal muscle, autophagy, signaling, plasma amino acid, exercise training, fasting, skeletal muscle",
author = "Dethlefsen, {Maja Munk} and L{\ae}rke Bertholdt and Anders Gudiksen and Tomasz Stankiewicz and Jens Bangsbo and {van Hall}, Gerrit and Peter Plomgaard and Henriette Pilegaard",
note = "CURIS 2018 NEXS 406",
year = "2018",
month = nov,
doi = "10.1152/japplphysiol.01146.2017",
language = "English",
volume = "125",
pages = "1609--1619",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "5",

}

RIS

TY - JOUR

T1 - Training state and skeletal muscle autophagy in response to 36 h of fasting

AU - Dethlefsen, Maja Munk

AU - Bertholdt, Lærke

AU - Gudiksen, Anders

AU - Stankiewicz, Tomasz

AU - Bangsbo, Jens

AU - van Hall, Gerrit

AU - Plomgaard, Peter

AU - Pilegaard, Henriette

N1 - CURIS 2018 NEXS 406

PY - 2018/11

Y1 - 2018/11

N2 - The present study aimed at investigating fasting-induced responses in regulators and markers of autophagy in vastus lateralis muscle from untrained and trained human subjects. Untrained and trained subjects (based on maximum oxygen uptake, muscle citrate synthase activity, and oxidative phosphorylation protein level) fasted for 36 h with vastus lateralis muscle biopsies obtained at 2, 12, 24, and 36 h after a standardized meal. Fasting reduced (P 0.05) skeletal muscle microtubule-associated protein-1A/1B light chain 3 (LC3)I, LC3II, and adaptor protein sequestosome 1/p62 protein content in untrained subjects only. Moreover, skeletal muscle RAC-alpha serine/threonine-protein kinase (AKT) Thr308, AMP-activated protein kinase (AMPK) Thr172, and Unc-51-like autophagy-activating kinase-1 (ULK1) Ser555 phosphorylation state, as well as Bcl-2-interacting coiled-coil protein-1 (Beclin1) and ULK1 Ser757 phosphorylation, was lower (P 0.05) in trained than untrained subjects during fasting. In addition, the plasma concentrations of several amino acids were higher (P 0.05) in trained than untrained subjects, and the plasma concentration profile of several amino acids was different in untrained and trained subjects during fasting. Taken together, these findings suggest that 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle and that training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle. NEW & NOTEWORTHY This study showed that skeletal muscle autophagy was only modestly affected in humans by 36 h of fasting. Hence, 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle, and training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle.

AB - The present study aimed at investigating fasting-induced responses in regulators and markers of autophagy in vastus lateralis muscle from untrained and trained human subjects. Untrained and trained subjects (based on maximum oxygen uptake, muscle citrate synthase activity, and oxidative phosphorylation protein level) fasted for 36 h with vastus lateralis muscle biopsies obtained at 2, 12, 24, and 36 h after a standardized meal. Fasting reduced (P 0.05) skeletal muscle microtubule-associated protein-1A/1B light chain 3 (LC3)I, LC3II, and adaptor protein sequestosome 1/p62 protein content in untrained subjects only. Moreover, skeletal muscle RAC-alpha serine/threonine-protein kinase (AKT) Thr308, AMP-activated protein kinase (AMPK) Thr172, and Unc-51-like autophagy-activating kinase-1 (ULK1) Ser555 phosphorylation state, as well as Bcl-2-interacting coiled-coil protein-1 (Beclin1) and ULK1 Ser757 phosphorylation, was lower (P 0.05) in trained than untrained subjects during fasting. In addition, the plasma concentrations of several amino acids were higher (P 0.05) in trained than untrained subjects, and the plasma concentration profile of several amino acids was different in untrained and trained subjects during fasting. Taken together, these findings suggest that 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle and that training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle. NEW & NOTEWORTHY This study showed that skeletal muscle autophagy was only modestly affected in humans by 36 h of fasting. Hence, 36-h fasting has effects on some mediators of autophagy in untrained human skeletal muscle, and training state influences the effect of fasting on autophagy signaling and on mediators of autophagy in skeletal muscle.

KW - Faculty of Science

KW - Fasting

KW - Exercise training

KW - Skeletal muscle

KW - Autophagy

KW - Signaling

KW - Plasma amino acid

KW - Autophagy

KW - Exercise training

KW - Fasting

KW - Plasma amino acid

KW - Signaling

KW - Skeletal muscle

KW - autophagy

KW - signaling

KW - plasma amino acid

KW - exercise training

KW - fasting

KW - skeletal muscle

U2 - 10.1152/japplphysiol.01146.2017

DO - 10.1152/japplphysiol.01146.2017

M3 - Journal article

C2 - 30161009

VL - 125

SP - 1609

EP - 1619

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 5

ER -

ID: 201903004