Thermostability of IFN-γ and IP-10 release assays for latent infection with Mycobacterium tuberculosis: A TBnet study
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Thermostability of IFN-γ and IP-10 release assays for latent infection with Mycobacterium tuberculosis : A TBnet study. / Blauenfeldt, Thomas; Wagner, Dirk; Aabye, Martine; Heyckendorf, Jan; Lange, Berit; Lange, Christoph; Ernst, Martin; Ravn, Pernille; Duarte, Raquel; Morais, Clara; Hoffmann, Matthias; Schoch, Otto D; Dominguez, Jose; Latorre, Irene; Ruhwald, Morten.
I: Tuberculosis (Edinburgh, Scotland), Bind 98, 05.2016, s. 7-12.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Thermostability of IFN-γ and IP-10 release assays for latent infection with Mycobacterium tuberculosis
T2 - A TBnet study
AU - Blauenfeldt, Thomas
AU - Wagner, Dirk
AU - Aabye, Martine
AU - Heyckendorf, Jan
AU - Lange, Berit
AU - Lange, Christoph
AU - Ernst, Martin
AU - Ravn, Pernille
AU - Duarte, Raquel
AU - Morais, Clara
AU - Hoffmann, Matthias
AU - Schoch, Otto D
AU - Dominguez, Jose
AU - Latorre, Irene
AU - Ruhwald, Morten
N1 - Copyright © 2015 Elsevier Ltd. All rights reserved.
PY - 2016/5
Y1 - 2016/5
N2 - INTRODUCTION: Interferon-γ (IFN-γ) inducible protein 10kD (IP-10) and IFN-γ release assays (IGRAs) are immunodiagnostic tests aiming to identify the presence of specific cellular immune responses, interpreted as markers for latent infection with Mycobacterium tuberculosis. Incubation at higher temperatures could affect IFN-γ and IP-10 responsiveness in order to improve the performance of IP-10 release assays and IGRAs.AIM: The aim of this study was to assess the robustness of whole blood based IP-10 release assay and IGRAs and the effect of hyper-thermic incubation (39 °C) on the diagnostic accuracy of IP-10 release assay and IGRAs.RESULTS: We included 65 patients with confirmed pulmonary tuberculosis and 160 healthy controls from 6 European centres collaborating in the TBnet. In patients, IP-10 responses increased 1.07 (IQR 0.90-1.36) fold and IFN-γ responses decreased 0.88 (IQR 0.57-1.02) fold, with 39 °C compared to 37 °C incubation temperature. At 37 °C IGRA sensitivity was 85% and IP-10 sensitivity was 82%, whereas specificity was 97% for both tests (p > 0.8). These minor changes observed as a result of hyper-thermic incubation were not sufficient to impact IGRA and IP-10 release assay test performance.CONCLUSION: The performance of IGRA and IP-10 release assays is robust despite variations in the incubation temperature between 37 °C and 39 °C.
AB - INTRODUCTION: Interferon-γ (IFN-γ) inducible protein 10kD (IP-10) and IFN-γ release assays (IGRAs) are immunodiagnostic tests aiming to identify the presence of specific cellular immune responses, interpreted as markers for latent infection with Mycobacterium tuberculosis. Incubation at higher temperatures could affect IFN-γ and IP-10 responsiveness in order to improve the performance of IP-10 release assays and IGRAs.AIM: The aim of this study was to assess the robustness of whole blood based IP-10 release assay and IGRAs and the effect of hyper-thermic incubation (39 °C) on the diagnostic accuracy of IP-10 release assay and IGRAs.RESULTS: We included 65 patients with confirmed pulmonary tuberculosis and 160 healthy controls from 6 European centres collaborating in the TBnet. In patients, IP-10 responses increased 1.07 (IQR 0.90-1.36) fold and IFN-γ responses decreased 0.88 (IQR 0.57-1.02) fold, with 39 °C compared to 37 °C incubation temperature. At 37 °C IGRA sensitivity was 85% and IP-10 sensitivity was 82%, whereas specificity was 97% for both tests (p > 0.8). These minor changes observed as a result of hyper-thermic incubation were not sufficient to impact IGRA and IP-10 release assay test performance.CONCLUSION: The performance of IGRA and IP-10 release assays is robust despite variations in the incubation temperature between 37 °C and 39 °C.
KW - Journal Article
U2 - 10.1016/j.tube.2015.04.013
DO - 10.1016/j.tube.2015.04.013
M3 - Journal article
C2 - 27156612
VL - 98
SP - 7
EP - 12
JO - Tuberculosis
JF - Tuberculosis
SN - 1472-9792
ER -
ID: 177120305