The tuatara genome reveals ancient features of amniote evolution

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Dokumenter

  • Neil J. Gemmell
  • Kim Rutherford
  • Stefan Prost
  • Marc Tollis
  • David Winter
  • J. Robert Macey
  • David L. Adelson
  • Alexander Suh
  • Terry Bertozzi
  • Jose H. Grau
  • Chris Organ
  • Paul P. Gardner
  • Matthieu Muffato
  • Mateus Patricio
  • Konstantinos Billis
  • Fergal J. Martin
  • Paul Flicek
  • Petersen, Bent
  • Lin Kang
  • Pawel Michalak
  • Thomas R. Buckley
  • Melissa Wilson
  • Yuanyuan Cheng
  • Hilary Miller
  • Ryan K. Schott
  • Melissa D. Jordan
  • Richard D. Newcomb
  • Jose Ignacio Arroyo
  • Nicole Valenzuela
  • Tim A. Hore
  • Jaime Renart
  • Valentina Peona
  • Claire R. Peart
  • Vera M. Warmuth
  • Lu Zeng
  • R. Daniel Kortschak
  • Joy M. Raison
  • Valeria Velasquez Zapata
  • Zhiqiang Wu
  • Didac Santesmasses
  • Marco Mariotti
  • Roderic Guigo
  • Shawn M. Rupp
  • Victoria G. Twort
  • Nicolas Dussex
  • Helen Taylor
  • Hideaki Abe
  • Donna M. Bond
  • James M. Paterson
  • Daniel G. Mulcahy
  • Ngatiwai Trust Board

The tuatara (Sphenodon punctatus)-the only living member of the reptilian order Rhynchocephalia (Sphenodontia), once widespread across Gondwana(1,2)-is an iconic species that is endemic to New Zealand(2,3). A key link to the now-extinct stem reptiles (from which dinosaurs, modern reptiles, birds and mammals evolved), the tuatara provides key insights into the ancestral amniotes(2,4). Here we analyse the genome of the tuatara, which-at approximately 5 Gb-is among the largest of the vertebrate genomes yet assembled. Our analyses of this genome, along with comparisons with other vertebrate genomes, reinforce the uniqueness of the tuatara. Phylogenetic analyses indicate that the tuatara lineage diverged from that of snakes and lizards around 250 million years ago. This lineage also shows moderate rates of molecular evolution, with instances of punctuated evolution. Our genome sequence analysis identifies expansions of proteins, non-protein-coding RNA families and repeat elements, the latter of which show an amalgam of reptilian and mammalian features. The sequencing of the tuatara genome provides a valuable resource for deep comparative analyses of tetrapods, as well as for tuatara biology and conservation. Our study also provides important insights into both the technical challenges and the cultural obligations that are associated with genome sequencing.

The approximately 5-Gb tuatara (Sphenodon punctatus) genome assembly provides a resource for analysing amniote evolution, and highlights the imperative for meaningful cultural engagement with Indigenous communities in genome-sequencing endeavours.

OriginalsprogEngelsk
TidsskriftNature
Vol/bind584
Sider (fra-til)403–409, +19
Antal sider26
ISSN0028-0836
DOI
StatusUdgivet - 2020

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